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Analysis of genes characterizing chronic thrombosis and associated pathways in chronic thromboembolic pulmonary

Shizhang Zhan1, Liu Wang2, Wenping Wang1

  • 1Bengbu Medical College, Bengbu, China.

Plos One
|March 7, 2024
PubMed

Insights

Researchers identified key genes like IL-1B and TNF involved in chronic thromboembolic pulmonary hypertension (CTEPH). These genes show potential for early diagnosis and novel treatments for CTEPH, a condition marked by blood vessel obstruction.

Area of Science:

  • * Genomics and bioinformatics
  • * Molecular biology
  • * Pulmonary vascular disease research

Background:

  • * Chronic thromboembolic pulmonary hypertension (CTEPH) involves thrombus fibrosis and vascular obstruction, critical for disease progression.
  • * Understanding key genes in chronic thrombosis is vital for CTEPH development insights.
  • * Identifying these genes aids early detection, prevention, and therapeutic strategies for CTEPH.

Purpose of the Study:

  • * To identify key genes contributing to chronic thrombosis in CTEPH.
  • * To explore potential diagnostic and therapeutic targets for CTEPH.
  • * To elucidate molecular pathways involved in CTEPH pathogenesis.

Main Methods:

  • * Analysis of Gene Expression Omnibus (GEO) dataset GSE130391 for CTEPH and Idiopathic Pulmonary Arterial Hypertension (IPAH) gene expression profiles.
  • * Screening of Differentially Expressed Genes (DEGs) followed by Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses.
  • * Application of Weighted Gene Co-Expression Network Analysis (WGCNA), STRING database for protein-protein interaction (PPI) network construction, and cytoHubba for hub gene identification.

Main Results:

  • * Identification of 924 DEGs between CTEPH and IPAH.
  • * Selection of the MEturquoise module and screening of 757 intersecting genes.
  • * Determination of ten hub genes: IL-1B, CXCL8, CCL22, CCL5, CCL20, TNF, IL-12B, JUN, EP300, and CCL4.

Conclusions:

  • * The identified hub genes (IL-1B, CXCL8, CCL22, CCL5, CCL20, TNF, IL-12B, JUN, EP300, CCL4) possess diagnostic and therapeutic potential for CTEPH.
  • * These genes are implicated in the chronic thrombosis characteristic of CTEPH.
  • * The NF-κB, AP-1 transcription factors, and TNF signaling pathways, highlighted by these pivotal genes, likely contribute to CTEPH progression.
Abstract

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