Correlational analysis of PLIN1 with inflammation in diabetic foot ulcer wounds

Mengting Wang ¹, Xiaoliang Cao ¹, Yanxing Shang ²

⁰Medical School, Nantong University, Nantong 226001, China; Department of Burn and Plastic Surgery, Affiliated Hospital 2 of Nantong University, The First People's Hospital of Nantong, Nantong 226001, China.

Diabetes Research and Clinical Practice +

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March 7, 2024

View abstract on PubMed

Summary

This study reveals that PLIN1 is elevated in diabetic foot ulcers (DFUs) and drives inflammation. Targeting PLIN1 offers a promising new therapeutic strategy for DFU treatment.

Area Of Science

Biomedical research
Molecular biology
Wound healing research

Background

Inflammation is a key factor in diabetic foot ulcer (DFU) pathogenesis.
Investigating novel molecular targets for DFU treatment is crucial.

Purpose Of The Study

To explore the role of PLIN1 in DFU.
To determine the association between PLIN1 expression and inflammation in DFUs.

Main Methods

Transcriptome sequencing and correlation analysis in DFU tissues.
Analysis of PLIN1 expression in patient tissues and serum.
In vitro studies using high-glucose stimulated keratinocytes (HaCaT cells).

Main Results

PLIN1 expression is upregulated in DFU tissues and serum.
PLIN1 positively correlates with inflammatory markers (leukocytes, neutrophils, monocytes, CRP, PCT, IL-1β, TNF-α).
Reduced PLIN1 expression decreases inflammatory mediators (iNOS, IL-1β, IL-6, IL-18, TNF-α) via the NF-κB pathway.

Conclusions

PLIN1 plays a significant role in mediating inflammatory damage in DFUs.
PLIN1 represents a potential therapeutic target for DFU management.

Keywords:

Diabetic foot ulcer HaCaT cells Inflammatory damage PLIN1