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Phenotypes for general behavior, activity, and body temperature in 3q29 deletion model mice
Daisuke Mori1,2,3, Ryosuke Ikeda4, Masahito Sawahata5,6
1Department of Psychiatry, Nagoya University Graduate School of Medicine, Nagoya, Japan. d-mori@med.nagoya-u.ac.jp.
Translational Psychiatry
|March 7, 2024
Summary
Whole genome analysis reveals copy number variations (CNVs) linked to psychiatric disorders. 3q29 deletion mice exhibit altered activity and body temperature, offering insights into schizophrenia and sleep disorder pathogenesis.
Area of Science:
- Neurogenetics
- Behavioral Neuroscience
- Chronobiology
Background:
- Copy number variations (CNVs) are implicated in psychiatric disorders, with 3q29 deletion showing a significant effect size.
- Existing 3q29 deletion mouse models primarily focus on behavioral phenotypes, neglecting circadian rhythm and sleep aspects crucial for neuropsychiatric disorders.
Purpose of the Study:
- To reevaluate the pathogenesis of 3q29 deletion by analyzing behavioral and temporal activity patterns in a recreated mouse model.
- To investigate novel insights into temporal activity and temperature fluctuations using a Nano-tag accelerometer chip in 3q29 deletion mice.
Main Methods:
- Generation of 3q29 deletion mice using genome editing technology.
- Comprehensive behavioral battery testing to assess common phenotypes.
- Implantation of Nano-tag (miniature acceleration sensor) for continuous monitoring of activity and body temperature.
Main Results:
- 3q29 deletion mice exhibited weight loss and behavioral phenotypes consistent with schizophrenia models, including increased rearing frequency.
- Nano-tag implantation revealed hypersensitive and rapid increases in activity and body temperature in 3q29 deletion mice during the lights-off phase.
Conclusions:
- The recreated 3q29 deletion mouse model confirms its utility for schizophrenia research.
- The observed temporal activity and temperature changes highlight potential links between 3q29 deletion, circadian rhythm disruption, and neuropsychiatric disorders, suggesting therapeutic avenues for associated sleep disturbances.
