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Single-cell Analysis Reveals One Cancer-associated Fibroblasts Subtype Linked To Metastasis In Breast Cancer: Mxra5 As A Potential Novel Marker For Prognosis.

Home
Research Domains
Biomedical And Clinical Sciences
Oncology And Carcinogenesis
Predictive And Prognostic Markers
Single-cell Analysis Reveals One Cancer-associated Fibroblasts Subtype Linked To Metastasis In Breast Cancer: Mxra5 As A Potential Novel Marker For Prognosis.

Related Experiment Video

A Mouse Model to Investigate the Role of Cancer-Associated Fibroblasts in Tumor Growth

A Mouse Model to Investigate the Role of Cancer-Associated Fibroblasts in Tumor Growth

Published on: December 22, 2020

Single-cell analysis reveals one cancer-associated fibroblasts subtype linked to metastasis in breast cancer: MXRA5 as a potential novel marker for prognosis.

Huifang Deng¹, Chengxu Wen¹, Shangxuan Jiang¹

¹Department of Thyroid and Breast Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan 430022, Hubei, China.

American Journal of Cancer Research

|March 8, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Keywords:

MXRA5 breast cancer cancer-associated fibroblasts metastasis

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Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles

Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles

Published on: May 14, 2021

Cancer-associated fibroblasts (CAFs) drive breast cancer metastasis. A specific subtype, FN1+CAF2, promotes tumor spread and macrophage polarization, with MXRA5 identified as a key regulatory gene.

Area of Science:

Oncology
Cell Biology
Cancer Research

Background:

Cancer-associated fibroblasts (CAFs) are key components of the tumor microenvironment in breast cancer.
CAF heterogeneity complicates understanding their specific roles in cancer progression.
Lack of specific markers hinders the investigation of CAF subpopulations' functions.

Purpose of the Study:

To identify distinct CAF phenotypes in breast cancer.
To elucidate the role of specific CAF subtypes in breast cancer metastasis.
To identify novel molecular markers associated with CAF-mediated metastasis.

Main Methods:

Single-cell RNA sequencing analysis of breast cancer datasets.
SCENIC analysis to identify transcription factors in CAFs.

single-cell analysis

Gene expression analysis (FN1, MXRA5) in patient tissues and cell models.

Functional assays involving CAF-mediated breast cancer cell migration.

Main Results:

Eight distinct CAF phenotypes were identified in breast cancer.
A specific subtype, FN1+CAF2, was associated with metastasis and macrophage polarization.
Elevated FN1 expression was observed in breast cancer stroma.
FN1 knockdown in CAFs reduced breast cancer cell migration.
Upregulated MXRA5 expression was found in breast cancer tissues and CAFs from patients with lymph node metastasis.

Conclusions:

The FN1+CAF2 subtype of CAFs promotes breast cancer metastasis.
MXRA5 is identified as a potential novel marker mediating CAF2's effects on metastasis.
Understanding CAF heterogeneity offers new therapeutic strategies for breast cancer metastasis.