Related Experiment Video
Updated: Jul 1, 2025

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
Published on: June 9, 2017
Measuring Ligand-bound Protein Complexes with Proximity Labeling: A Practical Guide
Pavitra Goyal1, Andrew J Tao1, Elizabeth J Mumby2
1Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA 92093.
Abstract:
Identifying the drug-target interactome of small molecule therapeutics is essential for understanding the full pharmacological effects of a compound. These therapies often induce changes within the cellular proteome, leading to unexpected consequences such as changes in the targets complexation state or off-target interactions between the compound and additional proteins. Currently, unbiased target-ID approaches are being used to embark on this task. Here we provide an overview of the strengths and limitations of these methods, and a practical step-by-step protocol for using the BioTAC system to assist with drug target and interactome ID.
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