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Updated: May 1, 2026

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Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry
Published on: November 20, 2014
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Convenient pre-separation method depends on negative charges suitable for glycan sequencing analysis
Shigemitsu Segawa1, Shunji Natsuka1
1Graduate School of Science and Technology, Niigata University, Niigata, Japan.
Bioscience, Biotechnology, and Biochemistry
|March 8, 2024
Abstract:
For precise structural analysis of complex glycans, it is necessary to fractionate glycans based on acidity prior to liquid chromatography-mass spectrometry analysis. Conventional fractionation using high-performance liquid chromatography is time consuming and not high throughput. Therefore, we developed a rapid method for the fractionation using a small cartridge column. This enables rapid multi-sample processing for glycan sequencing.

