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Southern Blot

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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
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Related Experiment Video

Updated: May 1, 2026

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

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Published on: November 20, 2014

Convenient pre-separation method depends on negative charges suitable for glycan sequencing analysis.

Shigemitsu Segawa¹, Shunji Natsuka¹

¹Graduate School of Science and Technology, Niigata University, Niigata, Japan.

Bioscience, Biotechnology, and Biochemistry

|March 8, 2024

Summary

A new rapid method for glycan fractionation using small cartridge columns improves glycan sequencing. This technique allows for faster multi-sample processing compared to conventional high-performance liquid chromatography methods.

Keywords:

anion exchange glycan sequencing mass spectrometry sialic acid

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Area of Science:

Analytical Chemistry
Biochemistry
Glycomics

Background:

Precise structural analysis of complex glycans is crucial for understanding biological processes.
Conventional glycan fractionation methods, such as high-performance liquid chromatography (HPLC), are often time-consuming and lack high throughput.
Efficient glycan separation is a prerequisite for accurate glycomic sequencing.

Purpose of the Study:

To develop a rapid and high-throughput method for glycan fractionation based on acidity.
To enable faster and more efficient glycan sequencing for structural analysis.
To overcome the limitations of conventional time-consuming fractionation techniques.

Main Methods:

Development of a novel glycan fractionation method utilizing a small cartridge column.
Application of the cartridge-based method for acidic glycan separation.
Integration of the rapid fractionation technique with liquid chromatography-mass spectrometry (LC-MS) analysis.

Main Results:

The developed method significantly reduces fractionation time compared to conventional HPLC.
The small cartridge column enables rapid multi-sample processing.
The method facilitates efficient glycan sequencing, improving overall workflow efficiency.

Conclusions:

The novel cartridge-based fractionation method offers a rapid and high-throughput solution for glycan analysis.
This approach streamlines glycan sequencing, making it more accessible for complex glycomic studies.
The developed technique represents a significant advancement in glycan structural analysis methodologies.