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Updated: May 7, 2026
Diffusion Tensor Magnetic Resonance Imaging in the Analysis of Neurodegenerative Diseases
Published on: July 28, 2013
Diffusion tensor imaging within the healthy cervical spinal cord: Within- participants reliability and measurement
Hussein Al-Shaari1, Jon Fulford2, C J Heales2
1Diagnostic Radiology Department, College of Applied Medical Sciences, Najran University, Najran 61441, Saudi Arabia; Department of Medical Imaging, Faculty of Health and Life Sciences, The University of Exeter, South Cloisters, University of Exeter, St Luke's Campus, Heavitree Road, Exeter EX1 2LU, UK.
Background:
Diffusion tensor imaging (DTI) is a promising technique for the visualization of the cervical spinal cord (CSC) in vivo. It provides information about the tissue structure of axonal white matter, and it is thought to be more sensitive than other MR imaging techniques for the evaluation of damage to tracts in the spinal cord.
Aim:
The purpose of this study was to determine the within-participants reliability and error magnitude of measurements of DTI metrics in healthy human CSC.
Methods:
A total of twenty healthy controls (10 male, mean age: 33.9 ± 3.5 years, 10 females, mean age: 47.5 ± 14.4 years), with no family history of any neurological disorders or a contraindication to MRI scanning were recruited over a period of two months. Each participant was scanned twice with an MRI 3 T scanner using standard DTI sequences. Spinal Cord Toolbox (SCT) software was used for image post-processing. Data were first corrected for motion artefact, then segmented, registered to a template, and then the DTI metrics were computed. The within-participants coefficients of variation (CV%), the single and average within-participants intraclass correlation coefficients (ICC) and Bland-Altman plots for WM, VC, DC and LC fractional anisotropy (FA), mean diffusivity (MD), axial diffusivity (AD), and radial diffusivity (RD) were determined for the cervical spinal cord (between the 2nd and 5th cervical vertebrae).
Results:
DTI metrics showed poor to excellent within-participants reliability for both single and average ICC and moderate to high reproducibility for CV%, all variation dependent on the location of the ROI. The BA plots showed good within-participants agreement between the scan-rescan values.
Conclusion:
Results from this reliability study demonstrate that clinical trials using the DTI technique are feasible and that DTI, in particular regions of the cord is suitable for use for the monitoring of degenerative WM changes.
