Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Editing02:23

RNA Editing

9.0K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.0K
Experimental RNAi02:15

Experimental RNAi

6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Longitudinal localization of leukaemic stem cells between the metaphysis and central marrow governs their behaviour.

Nature cell biology·2026
Same author

WITHDRAWN: A Druggable Tumor Suppressor and Leukemic Stem Cell Marker.

bioRxiv : the preprint server for biology·2026
Same author

Unrevealing the role of hypothalamic corticotropin-releasing hormone neurons in blood pressure regulation in hypertension.

Cardiovascular research·2025
Same author

Author Correction: SHP-1 inhibition targets leukaemia stem cells to restore immunosurveillance and enhance chemosensitivity by metabolic reprogramming.

Nature cell biology·2025
Same author

Microglia-Mediated Synaptic Dysfunction Contributes to Chemotherapy-Related Cognitive Impairment.

Journal of neurochemistry·2025
Same author

MicroRNA-145-5p inhibits the tumorigenesis of breast cancer through SENP2-regulated ubiquitination of ERK2.

Cellular and molecular life sciences : CMLS·2024

Related Experiment Video

Updated: Jul 1, 2025

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
09:16

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

Published on: September 1, 2019

7.6K

Limited dsRNA editing impedes leukemia stem cells.

Yi Pan1, XunLei Kang2

  • 1Division of Hematology and Oncology, Center for Precision Medicine, Department of Medicine, University of Missouri School of Medicine, Columbia, MO 65212, USA.

Trends in Cancer
|March 8, 2024
PubMed
Summary

Elevated RNA editing by adenosine deaminase acting on RNA (ADAR)-1 is key in T cell acute lymphoblastic leukemia (T-ALL) relapse. ADAR1 prevents cell death from double-stranded RNA sensing, impacting leukemia stem cell maintenance.

More Related Videos

Evaluation of Abnormal Growth-related Genes of Hematopoietic Stem and Progenitor Cells by Combining CRISPR/Cas9 Technology with Cell Counting
07:01

Evaluation of Abnormal Growth-related Genes of Hematopoietic Stem and Progenitor Cells by Combining CRISPR/Cas9 Technology with Cell Counting

Published on: May 2, 2025

122
Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models
08:14

Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models

Published on: October 3, 2019

12.2K

Related Experiment Videos

Last Updated: Jul 1, 2025

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
09:16

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

Published on: September 1, 2019

7.6K
Evaluation of Abnormal Growth-related Genes of Hematopoietic Stem and Progenitor Cells by Combining CRISPR/Cas9 Technology with Cell Counting
07:01

Evaluation of Abnormal Growth-related Genes of Hematopoietic Stem and Progenitor Cells by Combining CRISPR/Cas9 Technology with Cell Counting

Published on: May 2, 2025

122
Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models
08:14

Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models

Published on: October 3, 2019

12.2K

Area of Science:

  • Molecular Biology
  • Oncology
  • Immunology

Background:

  • Leukemia stem cells (LSCs) drive T cell acute lymphoblastic leukemia (T-ALL).
  • Understanding LSC generation and maintenance is vital for T-ALL therapy.
  • Mechanisms of T-ALL relapse require further elucidation.

Purpose of the Study:

  • To investigate the role of RNA editing in T-ALL.
  • To identify molecular features associated with T-ALL relapse.
  • To explore the function of adenosine deaminase acting on RNA (ADAR)-1 in T-ALL.

Main Methods:

  • Analysis of RNA editing patterns in T-ALL patient samples.
  • Assessment of ADAR1 expression and activity.
  • Investigation of ADAR1's role in apoptosis and double-stranded (ds)RNA sensing pathways.

Main Results:

  • Elevated ADAR1-mediated RNA editing is a hallmark of T-ALL relapse.
  • ADAR1 actively suppresses apoptosis induced by dsRNA.
  • This mechanism contributes to the survival of leukemia stem cells.

Conclusions:

  • ADAR1-mediated RNA editing is a critical factor in T-ALL relapse.
  • Targeting ADAR1 could offer a novel therapeutic strategy for T-ALL.
  • ADAR1's role in dsRNA sensing impacts LSC maintenance and survival.