Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Reconstruction of septin higher-order nano-size structures in ovarian cancer cells uncover susceptibility to the septin-targeting small molecule UR214-9.

bioRxiv : the preprint server for biology·2026

Same author

A sensitive orange fluorescent calcium ion indicator for imaging neural activity.

Nature communications·2026

Same author

A Genetically Encoded Calcium Ion Biosensor with an Exceptionally Large Ratiometric Response.

ACS sensors·2026

Same author

Reconstructing the global radiation of Turdidae (Aves: Passeriformes) using explicit geographic ranges under two different palaeogeographic scenarios.

Cladistics : the international journal of the Willi Hennig Society·2026

Same author

PinkyCaMP: an mScarlet-based calcium sensor with enhanced brightness, photostability and multiplexing capabilities.

Nature methods·2026

Same author

T-cell protrusions enable fast, localised initiation of chimeric antigen receptor signalling.

The EMBO journal·2026

Search research articles

Related Experiment Video

Updated: Jun 7, 2026

A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

Published on: September 2, 2013

An optogenetic method for the controlled release of single molecules.

Purba Kashyap¹, Sara Bertelli², Fakun Cao³

¹Institut für Chemie und Biochemie, Freie Universität Berlin, Berlin, Germany.

|March 8, 2024

Summary

Researchers developed an optogenetic system for precise, light-controlled release of single molecules in cells. This method enables single-molecule imaging and functional studies of proteins in live cells.

More Related Videos

Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons

Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons

Published on: March 28, 2016

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

Published on: March 22, 2018

Related Experiment Videos

Last Updated: Jun 7, 2026

A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

Published on: September 2, 2013

Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons

Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons

Published on: March 28, 2016

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

Published on: March 22, 2018

Area of Science:

Cell biology
Optogenetics
Molecular imaging

Background:

Studying protein function at the single-molecule level in live cells is challenging.
Controlling protein localization and release is crucial for understanding cellular processes.

Purpose of the Study:

To develop a novel optogenetic system for the controlled release of single molecules within cells.
To enable light-inducible delivery of functional proteins for single-molecule imaging and cellular function reconstitution.

Main Methods:

Utilized a photocleavable protein to confine soluble and transmembrane proteins to the Golgi apparatus.
Employed short light pulses to release proteins into the cytosol and plasma membrane in a dose-dependent manner.
Applied the system to reconstitute ion channel function and induce signaling pathways.

Main Results:

Achieved light-dependent delivery of functional proteins at levels suitable for single-molecule imaging.
Successfully reconstituted ion conductance by delivering BK and LRRC8 channels.
Induced NF-kB signaling in T lymphoblasts via controlled release of a signaling protein, demonstrating functional complex formation.

Conclusions:

Developed a versatile optogenetic tool for precise, single-molecule protein manipulation in live cells.
The system simplifies access to single-molecule microscopy and functional reconstitution of cellular pathways.
This method opens new avenues for investigating cellular function at the molecular level.