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Related Experiment Video

Updated: Jun 21, 2026

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
08:51

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

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Efficient and scalable gene delivery method with easily generated cationic carbon dots.

Manuel Algarra1, Elena Gonzalez-Muñoz2,3

  • 1INAMAT2-Institute for Advanced Materials and Mathematics, Department of Science, Public University of Navarra, 31006, Pamplona, Spain.

Biological Procedures Online
|March 8, 2024
PubMed
Summary

This study introduces cationic carbon dots (CCDs) for efficient gene delivery, improving cell viability and transfection rates. This scalable method offers a safer, more effective alternative for biomedical applications.

Keywords:
Carbon dotsCell transductionCell transfectionGene deliveryPolyethyleneimine (PEI)

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Area of Science:

  • Biotechnology
  • Materials Science
  • Cell Biology

Background:

  • Gene delivery faces challenges in cellular uptake, endosomal escape, and minimizing toxicity.
  • Biomedical gene delivery systems require scalability, ease of manufacture, and affordability.

Purpose of the Study:

  • To develop an efficient, safe, and scalable gene delivery method using carbon dots.
  • To create optimized cationic carbon dots (CCDs) for enhanced gene transfection.

Main Methods:

  • Synthesized cationic carbon dots (CCDs) by coating carbon dots with PEI via electrostatic binding.
  • Evaluated CCDs for cell viability and transfection efficiency compared to standard PEI polyplexes.
  • Tested CCDs in a challenging retroviral vector production protocol involving co-transfection of multiple plasmids.

Main Results:

  • CCDs demonstrated improved cell viability and a fourfold increase in transfection efficiency over PEI polyplexes.
  • The developed CCDs facilitated the challenging co-transfection of three plasmids for retroviral vector production.
  • High efficiency and functionality of gene delivery were confirmed by the production of infective retroviral particles.

Conclusions:

  • The developed CCDs represent an efficient, scalable, and biofunctional approach for gene delivery.
  • This method enhances transfection efficiency and cell viability, offering a promising alternative for gene therapy and vector production.
  • The study highlights the potential of engineered carbon dots in advancing biomedical gene delivery applications.