Related Experiment Video

Updated: Jul 1, 2025

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix
10:37

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix

Published on: October 20, 2021

2.9K

IL-18 and CD14 variants in chronic HBV predisposition: a case-control study with in silico analyses focused on

Mohammad Sarhadi1, Elham Pahlavani2, Niloufar Hosseini Razavi1

  • 1Cellular and Molecular Research Center, Research Institute of Cellular and Molecular Sciences in Infectious Diseases, Zahedan University of Medical Sciences, Zahedan, Iran.

Nucleosides, Nucleotides & Nucleic Acids
|March 9, 2024
PubMed

Insights

Genetic variations in IL-18 and CD14 influence chronic Hepatitis B virus (HBV) infection risk. The IL-18-rs187238 C>G variation acts as a protective factor against chronic HBV, while other combined genotypes increase risk.

Area of Science:

  • Immunogenetics
  • Hepatology
  • Bioinformatics

Background:

  • Hepatitis B virus (HBV) infection is a global health concern, potentially leading to severe liver diseases like cirrhosis and hepatocellular carcinoma.
  • Individual differences in immune response, influenced by genetic variations in cytokines, affect HBV clinical manifestations.
  • Immunogenetic profiling aids in understanding disease susceptibility and progression.

Purpose of the Study:

  • To investigate the association between specific genetic variations in the promoter regions of IL-18 (rs187238 C>G, rs1946518 T>G) and the intronic region of CD14 (rs2569190 A>G) and the risk of chronic HBV infection.
  • To analyze the functional impact of these genetic variations using bioinformatics tools.

Main Methods:

  • Genotyping of 400 individuals (200 cases, 200 controls) using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP).
  • Bioinformatic analysis to assess genomic conservation, transcription, splicing, and protein interactions.
  • Statistical analysis to determine the association between genotypes, haplotypes, and chronic HBV risk.

Main Results:

  • The IL-18-rs187238 C>G variation showed a protective effect against chronic HBV (OR=0.62, 95% CI: 0.46-0.83, p=0.002).
  • Specific combined genotypes (TG/CC/AA, TG/CC/AG, TT/CC/AG, GG/CC/AA) significantly increased chronic HBV risk (p<0.05).
  • IL-18 G/T and G/G haplotypes were associated with reduced risk (p<0.05). Bioinformatics suggested functional roles for IL-18-rs187238 C>G and CD14-rs2569190 A>G variations.

Conclusions:

  • The IL-18-rs187238 C>G polymorphism is a significant protective factor against chronic HBV infection.
  • Genetic variations in IL-18 and CD14 play a role in modulating the risk of chronic HBV.
  • Further research in larger, diverse populations is warranted to confirm these findings and explore underlying mechanisms.

Keywords:
CD14Hepatitis BRNA splicing factorsin silicointerleukin-18transcription factors

More Related Videos

Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System05:55

Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System

Published on: December 21, 2019

6.7K
Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors16:49

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors

Published on: July 16, 2012

11.6K

Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.3K
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.5K
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.3K