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Updated: Jul 1, 2025

Collection and Extraction of Saliva DNA for Next Generation Sequencing
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Collection and Extraction of Saliva DNA for Next Generation Sequencing

Published on: August 27, 2014

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Comparisons of different extraction methods and solvents for saliva samples

Lingli Fang1, Qiming Zhai1, Hua Zhang2

  • 1Chongqing Key Laboratory of Oral Disease and Biomedical Sciences, Stomatological Hospital of Chongqing Medical University, Chongqing, China.

Metabolomics : Official Journal of the Metabolomic Society
|March 9, 2024
PubMed
Summary

Protein precipitation (PPT) with acetonitrile or acetone is recommended for general saliva metabolite analysis. Liquid-liquid extraction (LLE) using chloroform and methanol is best for small hydrophobic compounds.

Keywords:
LC-MSMetabolomicsSalivary metabolitesSample preparation

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