SERPINB5 promotes colorectal cancer invasion and migration by promoting EMT and angiogenesis via the TNF-α/NF-κB pathway
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
View abstract on PubMed
Summary
This summary is machine-generated.High SERPINB5 expression in colorectal cancer (CRC) correlates with poor survival and promotes tumor invasion and angiogenesis by activating the TNF-α/NF-κB pathway.
Area Of Science
- Oncology
- Molecular Biology
- Cancer Research
Background
- Colorectal cancer (CRC) remains a significant health concern globally.
- Understanding the molecular mechanisms driving CRC progression is crucial for developing effective therapies.
Purpose Of The Study
- To investigate the role of SERPINB5 in colorectal cancer (CRC).
- To assess the clinicopathological significance of SERPINB5 expression in CRC patients.
- To elucidate the molecular pathways through which SERPINB5 influences CRC progression.
Main Methods
- Establishment of SERPINB5 knockdown and overexpression models in CRC cells.
- Bioinformatics analysis of SERPINB5 expression in CRC patient data.
- Assessment of cell migration, invasion, proliferation, and angiogenesis.
- Investigation of the TNF-α/NF-κB signaling pathway.
Main Results
- High SERPINB5 expression correlated with advanced N stage, lymphatic infiltration, and reduced progression-free survival.
- SERPINB5 overexpression upregulated TNF-α, p-NF-κB/p65, N-cadherin, MMP2, MMP9, and VEGFA, while downregulating E-cadherin.
- SERPINB5 enhanced CRC cell migration, invasion, and proliferation, and promoted angiogenesis.
- Inhibition of the TNF-α/NF-κB pathway reversed these effects.
Conclusions
- High SERPINB5 expression is a potential biomarker for poor prognosis in CRC patients.
- SERPINB5 promotes epithelial-mesenchymal transition (EMT) and angiogenesis via the TNF-α/NF-κB pathway.
- Targeting SERPINB5 or the TNF-α/NF-κB pathway may offer therapeutic strategies for CRC.
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
Related Concept Videos
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...