Related Experiment Video

Updated: Jul 1, 2025

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment
11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

1.5K

Daurisoline suppress glioma progression by inhibiting autophagy through PI3K/AKT/mTOR pathway and increases TMZ

Hai-Tang Yin1, Hui-Lu1, Ji-Hong Yang1

  • 1Department of Pharmacy, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou Province, PR China; College of Pharmacy, Guizhou Medical University, Guiyang, Guizhou Province, PR China.

Biochemical Pharmacology
|March 9, 2024
PubMed

Insights

Daurisoline (DAS) shows potent anti-glioma effects by inducing apoptosis and cell cycle arrest. It also enhances temozolomide (TMZ) efficacy by inhibiting autophagy, offering a new strategy for chemoresistant glioma.

Area of Science:

  • Neuro-oncology
  • Cancer Biology
  • Pharmacology

Background:

  • Glioma is a common primary malignant brain tumor.
  • Temozolomide (TMZ) is a standard chemotherapy with limited efficacy due to resistance.
  • Novel therapeutic agents are needed for effective glioma treatment.

Purpose of the Study:

  • To investigate the anti-glioma effects of Daurisoline (DAS).
  • To explore DAS's mechanism of action in glioma cells.
  • To evaluate DAS's potential to overcome temozolomide resistance.

Main Methods:

  • Cytotoxicity screening of 30 compounds using CCK-8 assay.
  • Assessment of apoptosis, cell cycle, migration, and invasion.
  • Analysis of the PI3K/AKT/mTOR pathway and autophagy.
  • In vivo efficacy study using a nude mice xenograft model.

Main Results:

  • Daurisoline (DAS) demonstrated potent cytotoxicity against glioma cells.
  • DAS induced apoptosis via caspase activation and G1 cell cycle arrest.
  • DAS suppressed glioma cell migration and invasion by regulating epithelial-mesenchymal transition.
  • DAS impaired late-stage autophagic flux through the PI3K/AKT/mTOR pathway.
  • DAS inhibited TMZ-induced autophagy, enhancing TMZ chemosensitivity.
  • In vivo studies confirmed DAS restrains glioma proliferation and improves TMZ chemosensitivity.

Conclusions:

  • Daurisoline (DAS) exhibits significant anti-glioma activity.
  • DAS enhances the efficacy of temozolomide (TMZ) in chemoresistant glioma models.
  • DAS represents a promising therapeutic candidate for glioma treatment.
Keywords:
ApoptosisAutophagyDaurisolineGliomaTemozolomide

More Related Videos

Method for Novel Anti-Cancer Drug Development using Tumor Explants of Surgical Specimens09:26

Method for Novel Anti-Cancer Drug Development using Tumor Explants of Surgical Specimens

Published on: July 29, 2011

16.7K
Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response Testing09:24

Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response Testing

Published on: June 9, 2016

9.1K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.8K
Drugs that Stabilize Microtubules01:15

Drugs that Stabilize Microtubules

Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...
2.0K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
3.6K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.8K