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Related Concept Videos

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Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Related Experiment Video

Updated: Jul 1, 2025

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease
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[Modeling T2 high severe asthma using human induced pluripotent stem cells (hiPSC)].

E Ahmed1, S Assou2, F Foisset2

  • 1Laboratoire d'immunologie muqueuse, centre VIB-UGent pour la recherche sur l'inflammation, université de Gand, 9000 Gand, Belgique; Département de maladies respiratoires, université de Montpellier, CHU de Montpellier, hôpital Arnaud de Villeneuve, Montpellier, France.

Revue Des Maladies Respiratoires
|March 9, 2024
PubMed
Summary

Severe asthma involves mucus plugs and airway obstruction. This study develops a human cell model to better understand these mechanisms and improve respiratory research for better severe asthma treatments.

Keywords:
Asthme sévèreBouchon muqueuxEosinophilEosinophileEpitheliumEpithéliumGalectin-10 crystalsGalectine 10Mucus plugsSevere asthma

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Area of Science:

  • Respiratory medicine
  • Cell biology
  • Stem cell research

Context:

  • Severe asthma is characterized by persistent airflow obstruction, often due to mucus plugs.
  • The exact molecular mechanisms driving this severe asthma endotype remain unclear.
  • Existing animal models do not fully replicate human airway epithelium, limiting research.

Purpose:

  • To establish a human in vitro model of severe asthma.
  • To recapitulate airway epithelium remodeling and mucus plug formation observed in vivo.
  • To facilitate a deeper understanding of severe asthma's molecular underpinnings.

Summary:

  • Researchers are developing an in vitro model using human-induced pluripotent stem cells (hiPSC) to mimic severe asthma.
  • This model aims to replicate the complex airway epithelium and mucus plug formation seen in patients.
  • The model will help elucidate the molecular mechanisms behind persistent airflow obstruction in severe asthma.

Impact:

  • This human in vitro model will advance respiratory research by overcoming limitations of animal models.
  • It provides a platform for studying severe asthma pathogenesis and identifying novel therapeutic targets.
  • Understanding mucus plug formation in severe asthma can lead to improved patient outcomes.