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Full-Length Single Protein Molecules Tracking and Counting in Thin Silicon Channels
Shilo Ohayon1, Liran Taib1, Navneet Chandra Verma1
1Department of Biomedical Engineering, Technion-IIT, Haifa, 3200003, Israel.
Advanced Materials (Deerfield Beach, Fla.)
|March 9, 2024
Summary
This study introduces a novel single-molecule protein sensing method for ultra-fast, antibody-free quantification. The technique enables parallel separation and tracking of intact proteins, advancing biomedical research and diagnostics.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Molecular Biology
Background:
- Single-molecule protein sensing is crucial for biomedical research.
- Current methods face challenges like protein fragmentation, antibody bias, and low throughput.
Purpose of the Study:
- To develop an ultra-fast, antibody-free single-molecule protein quantification method.
- To enable parallel separation and multi-dimensional analysis of intact proteins.
Main Methods:
- Proteins are labeled with chemo-selective dual amino-acid specific tags.
- Electrophoretic separation by mass/charge in a custom silicon channel.
- Tracking protein motion during migration for identification.
Main Results:
- Parallel analysis of thousands of proteins in minutes.
- Quantification of a cytokine panel for infection discrimination.
- Accurate quantification of Vascular Endothelial Growth Factor (VEGF) splice isoforms from serum.
Conclusions:
- The method provides non-destructive, antibody-free quantification of full-length intact proteins.
- Enables multi-dimensional molecular property analysis for protein identification.
- Offers a high-throughput solution for advanced proteomic studies and diagnostics.
