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Updated: Jun 23, 2026
Step-specific Sorting of Mouse Spermatids by Flow Cytometry
Published on: December 31, 2015
Microfluidic sperm sorting selects a subpopulation of high-quality sperm with a higher potential for fertilization
Nadia Sheibak1, Fatemehsadat Amjadi1,2, Amir Shamloo3,4
1Department of Anatomical Sciences, Reproductive Sciences and Technology Research Center, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Study Question:
Is a microfluidic sperm sorter (MSS) able to select higher quality sperm compared to conventional methods?
Summary Answer:
The MSS selects sperm with improved parameters, lower DNA fragmentation, and higher fertilizing potential.
What Is Known Already:
To date, the few studies that have compared microfluidics sperm selection with conventional methods have used heterogeneous study population and have lacked molecular investigations.
Study Design, Size, Duration:
The efficiency of a newly designed MSS in isolating high-quality sperm was compared to the density-gradient centrifugation (DGC) and swim-up (SU) methods, using 100 semen samples in two groups, during 2023-2024.
Participants/Materials, Setting, Methods:
Semen specimens from 50 normozoospermic and 50 non-normozoospermic men were sorted using MSS, DGC, and SU methods to compare parameters related to the quality and fertilizing potential of sperm. The fertilizing potential of sperm was determined by measurement of phospholipase C zeta (PLCζ) and post-acrosomal sheath WW domain-binding protein (PAWP) expression using flow cytometry, and the chromatin dispersion test was used to assess sperm DNA damage.
Main Results And The Role Of Chance:
In both normozoospermic and non-normozoospermic groups, the MSS-selected sperm with the highest progressive motility, PLCζ positive expression and PLCζ and PAWP fluorescence intensity the lowest non-progressive motility, and minimal DNA fragmentation, compared to sperm selected by DGC and SU methods (P < 0.05).
Limitation, Reasons For Caution:
The major limitations of our study were the low yield of sperm in the MSS chips and intentional exclusion of severe male factor infertility to yield a sufficient sperm count for molecular experiments; thus testing with severe oligozoospermic semen and samples with low count and motility is still required. In addition, due to ethical considerations, at present, it was impossible to use the sperm achieved from MSS in the clinic to assess the fertilization rate and further outcomes.
Wider Implications Of The Findings:
Our research presents new evidence that microfluidic sperm sorting may result in the selection of high-quality sperm from raw semen. This novel technology might be a key to improving clinical outcomes of assisted reproduction in infertile patients.
Study Funding/Competing Interest(S):
The study is funded by the Iran University of Medical Sciences and no competing interest exists.
Trial Registration Number:
N/A.
