Bioactive Wound Healing 3D Structure Based on Chitosan Hydrogel Loaded with Naringin/Cyclodextrin Inclusion...

Area of Science:

• Biomaterials Science
• Materials Chemistry
• Drug Delivery Systems

Background:

• Development of advanced wound healing materials is crucial for effective tissue regeneration.
• Chitosan hydrogels offer biocompatibility and biodegradability, making them suitable for wound dressings.
• Naringin (Nar) possesses antioxidant and anti-inflammatory properties beneficial for wound healing, but its delivery requires optimization.

Purpose of the Study:

• To fabricate and characterize a potent wound healing structure using a naringin/β-cyclodextrin-loaded chitosan hydrogel.
• To investigate the interactions and stability of the naringin/β-cyclodextrin inclusion complex within the chitosan hydrogel matrix.
• To evaluate the in vitro and in vivo efficacy of the developed hydrogel nanocomposite for wound healing applications.

Main Methods:

• In silico simulation studies (DFT-D, Monte Carlo) were employed to assess molecular interactions and complex formation.
• Fabrication of chitosan hydrogel nanocomposite loaded with naringin/β-cyclodextrin inclusion complexes.
• Characterization techniques including Zeta potential measurements, porosity analysis, degradation studies, MTT assay for biocompatibility, and in vivo wound contraction measurements.

Main Results:

• In silico studies confirmed the stable formation of a naringin/β-cyclodextrin inclusion complex with negative interaction energy (-93.626 kcal/mol).
• The chitosan/naringin/β-cyclodextrin nanocomplex exhibited significant interaction energy (-1.423 × 103 kcal/mol), indicating a stable structure.
• Characterization confirmed the inclusion complex formation, with increased negative Zeta potential (-17.60 ± 2.34 mV). The hydrogel showed high porosity (70-90%), controlled degradation, biocompatibility (MTT assay), and accelerated wound closure in animal models compared to the control group.

Conclusions:

• The developed chitosan hydrogel containing a naringin/β-cyclodextrin inclusion nanocomplex is a stable and bioactive wound healing material.
• The nanocomposite hydrogel demonstrates excellent biocompatibility and promotes faster wound healing.
• This novel 3D structure holds significant potential as an effective material for advanced wound dressing applications.