JoVE - Journal of Visualized Experiments
JoVE Visualize
Contact Us

miR-143 promotes cell proliferation, invasion and migration via directly binding to BRD2 in lens epithelial cells

  • 0Department of Ophthalmology, China-Japan Friendship Hospital Beijing 100029, China.
American Journal of Translational Research +

|

March 11, 2024

|

March 11, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

MicroRNA-143 (miR-143) suppresses lens epithelial cell growth and movement by targeting Bromodomain containing 2 (BRD2). This finding offers a potential new therapy for cataract patients.

Area Of Science

  • Ophthalmology
  • Molecular Biology
  • Genetics

Background

  • Cataract is a leading cause of global blindness.
  • Understanding the molecular mechanisms of lens epithelial cell behavior is crucial for developing effective treatments.

Purpose Of The Study

  • To investigate the role of microRNA-143 (miR-143) in human lens epithelial cells.
  • To explore the relationship between miR-143 and Bromodomain containing 2 (BRD2) in the context of cataract.

Main Methods

  • Comparative analysis of miRNA expression profiles in cataract and myopia.
  • Real-time quantitative PCR (RT-qPCR) to quantify miR-143 and BRD2 levels.
  • In vitro assays (Transwell, wound healing, dual-luciferase) to assess cell function and gene regulation.
  • Gene silencing techniques (siRNA-BRD2, miR-143 inhibitors) were employed.

Main Results

  • MiR-143 was significantly upregulated in cataract compared to myopia.
  • MiR-143 inhibition reduced proliferation, invasion, and migration of lens epithelial cells.
  • BRD2 was confirmed as a direct target of miR-143, and its knockdown promoted cell proliferation and migration, partially reversing miR-143 inhibitor effects.

Conclusions

  • MiR-143 acts as a tumor suppressor in lens epithelial cells by inhibiting proliferation, invasion, and migration.
  • The miR-143/BRD2 pathway represents a potential therapeutic target for managing cataract progression.

Keywords:

Related Concept Videos

The Retinoblastoma Gene 01:20

4.1K

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...

Metastasis 02:30

5.5K

Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...

Mitogens and the Cell Cycle 02:38

6.5K

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...

Abnormal Proliferation 02:23

4.5K

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

Cell Motility through Blebbing 01:16

1.9K

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...