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Conductometric immunosensor for specific Escherichia coli O157:H7 detection on chemically funcationalizaed
Muhammad Nur Afnan Uda1, Alaa Kamal Yousif Dafhalla2, Thikra S Dhahi3
1Fakulti Kejuruteraan, Universiti Malaysia Sabah, 88400, Kota Kinabalu, Sabah, Malaysia.
Heliyon
|March 11, 2024
Summary
A new conductometric immunosensor detects Escherichia coli O157:H7, a foodborne pathogen, with high sensitivity. This rapid detection method offers potential for food safety and disease diagnosis.
Area of Science:
- Biosensor technology
- Electrochemical sensing
- Foodborne pathogen detection
Background:
- Escherichia coli O157:H7 is a significant cause of foodborne illness.
- Accurate and rapid detection of this pathogen is crucial for public health.
Purpose of the Study:
- To develop a conductometric immunosensor for detecting Escherichia coli O157:H7.
- To evaluate the sensor's performance in terms of sensitivity, selectivity, and detection range.
Main Methods:
- Fabrication of an interdigitated electrode modified with anti-Escherichia coli O157:H7 aptamer.
- Utilizing a conductometric sensing approach for signal transduction.
- Immobilization of the aptamer using 3-Aminopropyl triethoxysilane.
Main Results:
- The immunosensor demonstrated high sensitivity with a detection limit of 1 fM.
- A wide detection range from 1 fM to 1 nM was achieved.
- The sensor provided rapid and selective detection of Escherichia coli O157:H7.
Conclusions:
- The developed conductometric immunosensor offers a simple, fast, and highly sensitive method for detecting Escherichia coli O157:H7.
- This biosensor has potential applications in food safety, environmental monitoring, and disease diagnosis.
- The sensor's ability for rapid sample processing and real-time biomolecular interaction analysis is advantageous.
