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Updated: Jul 1, 2025

Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells
Published on: May 16, 2019
Strategies for Inducing and Validating Zinc Deficiency and Zinc Repletion
Tara-Yesomi Wenegieme1,2, Dalia Elased1, Kelia E McMichael1
1Department of Neuroscience, Cell Biology and Physiology, Wright State University, Dayton, OH 45435 USA.
Abstract:
Given the growing interest in the role of zinc in the onset and progression of diseases, there is a crucial demand for reliable methods to modulate zinc homeostasis. Using a dietary approach, we provide validated strategies to alter whole-body zinc in mice, applicable across species. For confirmation of zinc status, animal growth rates as well as plasma and urine zinc levels were evaluated. The accessible and cost-effective methodology outlined will increase scientific rigor, ensuring reproducibility in studies exploring the impact of zinc deficiency and repletion on the onset and progression of diseases.
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