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Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
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CD44 facilitates adhesive interactions in airineme-mediated intercellular signaling
Raquel Lynn Bowman1, Jiyea Kim1, Dae Seok Eom1
1Department of Developmental and Cell Biology, University of California, Irvine, CA 92617, USA.
Biorxiv : the Preprint Server for Biology
|March 11, 2024
Summary
Cellular airinemes mediate long-distance Notch signaling for pigment patterns. Macrophage interaction with CD44 glycoprotein on airineme vesicles is essential for this signaling and pattern formation.
Area of Science:
- Cell Biology
- Developmental Biology
- Molecular Biology
Background:
- Specialized cellular protrusions, such as airinemes, facilitate intercellular communication.
- Airinemes are crucial for pigment pattern formation in zebrafish by mediating long-distance Notch signaling.
- Macrophages interact with airineme vesicles, but the molecular details of this interaction are unclear.
Purpose of the Study:
- To elucidate the molecular mechanisms underlying macrophage interaction with airineme vesicles.
- To investigate the role of CD44 in airineme-mediated signaling and pigment pattern formation.
Main Methods:
- High-resolution live imaging
- Genetic in vivo manipulations in zebrafish
- In vitro adhesion assays
Main Results:
- Adhesive interactions involving the extracellular domain of CD44 between macrophages and airineme vesicles are critical for airineme signaling.
- Mutants lacking the CD44 extracellular domain showed reduced airineme extension and pigment pattern defects.
- CD44-mediated adhesion is essential for pulling and delivering airineme vesicles to target cells.
Conclusions:
- CD44-mediated adhesion is a key molecular interaction facilitating long-range intercellular signaling via airinemes.
- This study reveals the crucial role of cell adhesion molecules in coordinating developmental processes through specialized cellular communication.
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