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Related Experiment Video

Updated: Jul 1, 2025

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Published on: December 28, 2011

High-Resolution Spatial Transcriptomic Atlas of Mouse Soleus Muscle: Unveiling Single Cell and Subcellular

Jer-En Hsu¹, Lloyd Ruiz¹, Yongha Hwang^1,2

¹Department of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, MI, USA.

Biorxiv : the Preprint Server for Biology

|March 11, 2024

Summary

This summary is machine-generated.

This study maps gene expression in mouse skeletal muscle with submicron resolution using Seq-Scope. It reveals cellular and subcellular details, enhancing understanding of muscle aging and injury responses.

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Last Updated: Jul 1, 2025

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

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Published on: December 28, 2011

Author Spotlight: Investigating mRNA Spatial Distribution in Drosophila Muscle Tissue

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Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

Published on: December 12, 2016

Area of Science:

Muscle Biology
Genomics
Biotechnology

Background:

Skeletal muscle is vital for movement and metabolism, but detailed spatial gene expression mapping is limited by current technologies.
Understanding muscle aging and injury requires high-resolution spatial transcriptomic data.

Approach:

Employed Seq-Scope technology for submicron spatial resolution transcriptome-wide analysis.
Applied the technique to mouse soleus muscle under healthy and denervated conditions.
Examined gene expression in muscle fibers, associated cell types, and subcellular structures.

Key Points:

Detailed spatial gene expression profiles of muscle fibers, including hybrid fibers and postsynaptic nuclei.
Identified localized gene expression patterns associated with muscle injury and denervation.
Revealed cellular and subcellular heterogeneity within skeletal muscle tissue.

Conclusions:

The study provides unprecedented spatial insights into skeletal muscle transcriptomic diversity.
Findings advance understanding of denervation's impact on muscle, relevant to aging and disease.
Paves the way for novel therapeutic strategies targeting muscle health and age-related decline.