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Somatic to iPS Cell Reprogramming

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...

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Related Experiment Video

Updated: Jul 1, 2025

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Published on: July 30, 2016

Selecting Monoclonal Cell Lineages from Somatic Reprogramming Using Robotic-Based Spatial-Restricting Structured

Xueping Chen¹, Ke Fan¹, Jun Lu^1,2

¹Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, People's Republic of China.

Research (Washington, D.C.)

|March 11, 2024

Summary

This summary is machine-generated.

A novel robotic platform automates induced pluripotent stem cell (iPSC) selection using radial shear flow. This non-invasive, label-free system enhances iPSC purity and streamlines regenerative medicine development.

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Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

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Published on: November 27, 2017

Area of Science:

Biotechnology
Stem Cell Biology
Regenerative Medicine

Background:

Somatic cell reprogramming generates induced pluripotent stem cells (iPSCs) for personalized medicine.
Reprogramming leads to cellular heterogeneity, making monoclonal iPSC harvesting labor-intensive and reducing reproducibility.
Current methods for isolating iPSCs are inefficient and require extensive manual labor.

Purpose of the Study:

To develop an automated robotic platform for efficient and non-invasive monoclonal induced pluripotent stem cell (iPSC) colony selection.
To improve the purity and reproducibility of iPSC generation for regenerative medicine applications.
To reduce the labor and time associated with iPSC isolation and expansion.

Main Methods:

Development of a robotic platform utilizing pin-tip microstructures to manipulate radial shear flow for non-invasive, label-free iPSC colony selection.
Integration of automated somatic cell reprogramming culturing, medium changes, and high-content imaging.
Implementation of a dual flow-based iPSC selection process for enhanced purity and elimination of single-cell subcloning.

Main Results:

The automated system achieved monoclonal iPSC colony selection in approximately 1 second.
The robotic system can process approximately 24 somatic cell reprogramming tasks in parallel over 50 days.
iPSCs generated via the robotic approach showed 3.7 times greater purity compared to conventional manual methods.
Automated iPSCs maintained typical pluripotent transcriptional profiles, differentiation potential, and karyotypes.

Conclusions:

The developed robotic platform offers a promising solution for automated isolation and purification of iPSCs.
This technology can accelerate the development of personalized medicines by streamlining iPSC generation.
The non-invasive, label-free, and high-throughput nature of the system addresses key challenges in iPSC research and application.