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Related Experiment Videos

Leukocyte complement: interleukin-like properties of factor Bb.

S Hirani, D S Fair, R A Papin

    Cellular Immunology
    |May 1, 1985
    PubMed
    Summary
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    Factor B (Factor Bb), a complement protein, acts like a monokine or lymphokine. It stimulates mononuclear cells and induces lysosomal enzyme release from macrophages, suggesting a role in immune responses and autoimmune diseases.

    Area of Science:

    • Immunology
    • Complement System
    • Cellular Immunology

    Background:

    • The activated form of Factor B (Factor Bb) is known to stimulate monocyte spreading and killing of pathogens.
    • Native Factor B is produced by murine macrophages, and Factor Bb can stimulate lymphocyte blastogenesis.
    • The potential monokine or lymphokine-like properties of Factor Bb warrant investigation.

    Purpose of the Study:

    • To evaluate the potential monokine/lymphokine-like activities of Factor Bb.
    • To quantify Factor B release from human mononuclear cells stimulated with phytohemagglutinin (PHA).
    • To investigate the effects of purified Factor Bb on lysosomal enzyme release from murine macrophages.

    Main Methods:

    • Development of a radioimmunoassay to measure Factor B antigen levels in cell culture supernatants.

    Related Experiment Videos

  • Culture of human peripheral blood mononuclear cells with and without PHA stimulation in serum-free medium.
  • In vitro study using murine peritoneal exudate macrophages treated with purified Factor Bb.

    • Main Results:

    • PHA stimulation significantly increased Factor B antigen release from human mononuclear cells (to 1-3 X 10(-8) M/liter).
    • The molecular size of released Factor B was consistent with Factor Bb (50-65 kDa).
    • Factor Bb induced dose- and time-dependent release of lysosomal hydrolases (hexosaminidase, beta-glucuronidase) from murine macrophages.

    Conclusions:

    • Factor B is synthesized, released, and activated during mononuclear cell culture, exhibiting lymphokine/monokine-like activity.
    • Factor Bb triggers the release of lysosomal enzymes from macrophages, potentially contributing to pathological processes in autoimmune diseases.
    • Factor B/Bb can be considered a lymphokine, monokine, or interleukin based on its observed functions.