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Related Concept Videos

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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Related Experiment Video

Updated: May 12, 2026

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
08:25

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

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Plant Functional Genomics Based on High-Throughput CRISPR Library Knockout Screening: A Perspective.

Jianjie He1,2, Can Zeng1,2, Maoteng Li1,2

  • 1Department of Biotechnology College of Life Science and Technology Huazhong University of Science and Technology Wuhan 430074 China.

Advanced Genetics (Hoboken, N.J.)
|March 11, 2024
PubMed
Summary
This summary is machine-generated.

This study explores CRISPR-Cas9 gene editing for plant functional genomics. It highlights CRISPR libraries as powerful tools for high-throughput gene knockout and discovering functions in uncharacterized plant DNA sequences.

Keywords:
CRISPR libraryfunctional genomicshigh throughputknockoutmutant collection

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Last Updated: May 12, 2026

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Area of Science:

  • Plant Biology
  • Genomics
  • Molecular Biology

Background:

  • Post-genome era plant biology focuses on gene function annotation.
  • Established mutant collections have advanced functional genomics.
  • Many plant genome functions and regulatory networks remain unknown.

Purpose of the Study:

  • To compare current plant functional genomics methods.
  • To discuss CRISPR-Cas9 strategies for multi-targeted DNA perturbation and overcoming gene redundancy.
  • To summarize CRISPR library applications in plants for gene knockout and function discovery.

Main Methods:

  • Comparative analysis of plant functional genomics techniques.
  • CRISPR-Cas9 system for targeted DNA modifications.
  • CRISPR library screening for high-throughput analysis.

Main Results:

  • CRISPR-Cas9 offers versatile genome manipulation for functional studies.
  • Multi-targeted strategies effectively address gene redundancy.
  • CRISPR libraries enable efficient gene knockout and function discovery.

Conclusions:

  • CRISPR technology significantly enhances plant functional genomics research.
  • CRISPR library screening is a key strategy for exploring uncharacterized plant DNA.
  • Future work should focus on optimizing CRISPR screening for broader genomic applications.