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Published on: December 19, 2019
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Cell proliferation and apoptosis in canine oral papillomatosis
Emin Karakurt1, Nuvit Coskun2, Ugur Aydin3
1Department of Pathology, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye.
Summary
Canine oral papillomatosis, caused by canine papillomavirus-1, shows high cell proliferation and a mutant p53 gene. Despite this, apoptosis is favored, preventing malignant transformation in affected dogs.
Area of Science:
- Veterinary Pathology
- Oncology
- Immunohistochemistry
Background:
- Canine oral papillomatosis is a common condition caused by canine papillomavirus-1.
- Understanding the molecular mechanisms of tumor development is crucial for canine health.
Purpose of the Study:
- To evaluate cell proliferation, p53 protein levels, and the apoptotic index in canine oral papillomatosis.
- To investigate the role of p53 gene mutation and the Bax/Bcl-2 ratio in tumor development.
- To determine the apoptotic pathways involved in canine oral papillomatosis.
Main Methods:
- Immunohistochemical analysis using the avidin-biotin peroxidase method.
- Assessment of cell proliferation markers.
- Evaluation of p53, Bax, and Bcl-2 protein expression.
Main Results:
- Canine oral papillomatosis cases exhibited a high cell proliferation index.
- All cases tested positive for a mutant p53 gene.
- The Bax/Bcl-2 ratio favored the pro-apoptotic Bax gene, indicating apoptosis activation.
- Apoptosis occurred via both caspase-dependent and caspase-independent pathways.
- No malignant transformation was observed in any of the six cases, despite extensive lesions.
Conclusions:
- Canine oral papillomatosis is characterized by increased cell proliferation and p53 gene mutation.
- The apoptotic machinery remains functional, preventing malignant progression.
- Further research into therapeutic strategies targeting these pathways may be beneficial.
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