Effect of gastrointestinal digestion on the stability, antioxidant activity, and Caco-2 cellular transport of pigmented grain polyphenols
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View abstract on PubMed
Summary
This summary is machine-generated.Pigmented grain polyphenols show reduced content after digestion, but purple rice retains significant antioxidant activity. Some compounds like protocatechuic acid crossed the intestinal barrier.
Area Of Science
- Food Science
- Nutritional Biochemistry
- Pharmacology
Background
- Grain polyphenols offer health benefits but their digestive stability and absorption are not fully understood.
- Understanding polyphenol fate is crucial for harnessing their bioactivity.
Purpose Of The Study
- To investigate the digestive stability and intestinal absorption of pigmented grain polyphenols.
- To analyze the impact of simulated digestion on phenolic content and antioxidant activity.
Main Methods
- Simulated gastric and intestinal digestion of purple rice, purple barley, purple wheat, and blue wheat extracts.
- Caco-2 cellular transport assays to assess intestinal absorption.
- Phenolic profiling and antioxidant activity assays using UHPLC-ABTS•+.
Main Results
- Total phenolic content decreased post-digestion; purple rice extract retained 79% phenolic content and 31% ABTS•+ antioxidant activity.
- Antioxidant activity was highest during the gastric phase, with diminished dominant compounds after intestinal digestion.
- Protocatechuic acid, vanillic acid, apigenin, and chrysoeriol were transported across the intestinal barrier.
Conclusions
- Pigmented grain polyphenols exhibit varying stability and bioactivity after simulated digestion.
- Purple rice demonstrates superior retention of phenolic content and antioxidant capacity.
- Specific phenolic compounds are absorbed, indicating potential bioavailability and therapeutic relevance.
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