Related Experiment Video

Updated: Jul 1, 2025

Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis
08:55

Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis

Published on: August 7, 2018

10.9K

Necroptosis and Its Involvement in Various Diseases

Hiroyasu Nakano1

  • 1Department of Biochemistry, Faculty of Medicine, Toho University School of Medicine, Tokyo, Japan. hiroyasu.nakano@med.toho-u.ac.jp.

Advances in Experimental Medicine and Biology
|March 12, 2024
PubMed

Insights

Necroptosis, a regulated cell death, causes early plasma membrane rupture and releases danger-associated molecular patterns (DAMPs), driving inflammation. Understanding necroptosis mechanisms may offer new therapeutic strategies for related diseases.

Area of Science:

  • Cellular biology
  • Immunology
  • Pathology

Background:

  • Necroptosis is a regulated form of cell death.
  • It is implicated in various pathological conditions.
  • Necroptosis involves early plasma membrane rupture, leading to inflammation via danger-associated molecular patterns (DAMPs) release.

Purpose of the Study:

  • To elucidate the mechanisms of plasma membrane rupture (PMR) in necroptosis.
  • To understand the release of danger-associated molecular patterns (DAMPs).
  • To explore the therapeutic potential of targeting necroptosis signaling pathways.

Main Methods:

  • Review of recent studies on necroptosis mechanisms.
  • Analysis of stimuli inducing necroptosis (e.g., TNF, viral infection).
  • Investigation of necrosome formation and its role in PMR.

Main Results:

  • Necroptosis triggers early plasma membrane rupture, distinct from apoptosis.
  • The release of intracellular contents (DAMPs) from ruptured cells induces strong inflammatory responses.
  • Mutations in necroptosis-related genes are linked to hereditary autoinflammatory syndromes.

Conclusions:

  • Necroptosis is a key driver of inflammation through DAMPs release.
  • Targeting necroptosis pathways presents a potential therapeutic avenue for diseases associated with this cell death process.
Keywords:
ApoptosisDanger-associated molecular patternLive cell imaging for secretion activityMixed lineage kinase domain-like proteinNecroptosisPlasma membrane ruptureReceptor-interacting protein kinase 1

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