Related Experiment Video

Updated: Jul 1, 2025

LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring
08:45

LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring

Published on: November 17, 2018

13.4K

Nobiletin alleviates atherosclerosis by inhibiting lipid uptake via the PPARG/CD36 pathway

Heng Wang1, Qinqin Tian1, Ruijing Zhang2

  • 1Department of Vascular Surgery, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, China.

Lipids in Health and Disease
|March 12, 2024
PubMed

Insights

Nobiletin (NOB) alleviates atherosclerosis by inhibiting lipid uptake. This study identified key genes and pathways, demonstrating NOB

Area of Science:

  • Biochemistry and Molecular Biology
  • Pharmacology
  • Cardiovascular Research

Background:

  • Atherosclerosis (AS) is a chronic inflammatory disease driven by lipid accumulation and macrophage infiltration.
  • Nobiletin (NOB) shows potential in alleviating AS, but its precise mechanism requires elucidation.

Purpose of the Study:

  • To elucidate the molecular mechanisms by which Nobiletin (NOB) exerts its anti-atherosclerotic effects.
  • To identify key genes, pathways, and cellular processes involved in NOB's action against atherosclerosis.

Main Methods:

  • Comprehensive bioinformatic analyses including target prediction and pathway enrichment (GO, KEGG).
  • In vitro assays (cell scratch, oil red O, ELISA, flow cytometry, qRT-PCR, Western blotting) and in vivo AS mouse models.
  • Drug binding site prediction (DeepSite, AutoDock) and immune cell infiltration analysis (CIBERSORT).

Main Results:

  • Identified 141 potential target genes for NOB intervention in atherosclerosis.
  • Highlighted lipid metabolism and atherosclerosis, and fluid shear stress pathways as dominant.
  • Confirmed NOB inhibits lipid uptake and foam cell formation in vitro, and attenuates AS lesions, lipid accumulation, and macrophage infiltration in vivo, linked to PPARG/CD36 signaling.

Conclusions:

  • Nobiletin (NOB) effectively alleviates atherosclerosis.
  • The mechanism involves the inhibition of lipid uptake through the Peroxisome proliferator-activated receptor gamma (PPARG)/CD36 pathway.
  • NOB demonstrates therapeutic potential for atherosclerosis treatment.
Abstract

Keywords:
AtherosclerosisInflammationMacrophagocyteNetwork pharmacologyNobiletinPPARG/CD36 pathway

More Related Videos

Differential Effects of Lipid-lowering Drugs in Modulating Morphology of Cholesterol Particles09:15

Differential Effects of Lipid-lowering Drugs in Modulating Morphology of Cholesterol Particles

Published on: November 10, 2017

14.6K
Author Spotlight: Establishing MASLD Cell Models for Investigating Disease Mechanisms and the Lipid-Lowering Effects of Koumiss07:03

Author Spotlight: Establishing MASLD Cell Models for Investigating Disease Mechanisms and the Lipid-Lowering Effects of Koumiss

Published on: July 19, 2024

888

Related Concept Videos

Lipid-Lowering Drugs: Statins and Miscellaneous Agents01:20

Lipid-Lowering Drugs: Statins and Miscellaneous Agents

Hyperlipidemia, a medical condition often referred to as high cholesterol, is characterized by abnormally elevated levels of lipids in the bloodstream. When present in excess, these lipids, specifically cholesterol and triglycerides, can lead to serious health complications, often involving cardiovascular diseases. Illnesses like atherosclerosis, heart attacks, and pancreatitis have all been linked to untreated hyperlipidemia. This means controlling and regulating cholesterol and triglyceride...
661
Transducer Mechanism: Nuclear Receptors01:31

Transducer Mechanism: Nuclear Receptors

Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
1.3K
Treatment for Pulmonary Arterial Hypertension: Receptor Tyrosine Kinase Inhibitors and Calcium Channel Blockers01:26

Treatment for Pulmonary Arterial Hypertension: Receptor Tyrosine Kinase Inhibitors and Calcium Channel Blockers

Receptor tyrosine kinase inhibitors (TKIs) and calcium channel blockers (CCBs) are two critical categories of drugs employed in the treatment of pulmonary artery hypertension (PAH). PAH is a disease that causes high blood pressure in the pulmonary arteries, resulting in chest pain, fatigue, and shortness of breath.
TKIs, such as imatinib (Gleevec), are particularly effective in tackling the growth and mitogenic factors that become upregulated in PAH patients. These factors contribute to the...
166
Adrenergic Antagonists: ɑ and β-Receptor Blockers01:31

Adrenergic Antagonists: ɑ and β-Receptor Blockers

Third-generation β-blockers, such as labetalol and carvedilol, represent a significant advancement in managing cardiovascular conditions. Unlike conventional β-blockers, which can induce peripheral vasoconstriction, third-generation drugs block α1 adrenoceptors. This promotes vasodilation through several mechanisms, such as increased nitric oxide production, inhibition of calcium ion entry, opening of potassium ion channels, and antioxidant action. Labetalol, for instance, is...
447
Cholesterol: Significance and Regulation01:29

Cholesterol: Significance and Regulation

Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
544