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Viruses with RNA Genomes

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...

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Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

Procedures and potential pitfalls for constructing a bee-infecting RNA virus clone.

Wei-Fone Huang¹, Runlin Li¹, Lang Jin¹

¹College of Animal Science (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou, China.

Frontiers in Insect Science

|March 12, 2024

Summary

Creating infectious cDNA clones for bee-infecting RNA viruses is crucial for understanding pollinator decline. This article details the process and challenges, aiming to advance reverse-genetic studies of these vital insect viruses.

Keywords:

DWV SBV honey bees picornalike virus reversed genetics

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Area of Science:

Virology
Entomology
Ecology

Background:

Honey bee populations face decline due to various stressors, including viral infections.
Many honey bee viruses are single positive-stranded RNA viruses that can affect other pollinators and synergize with other stressors.
Understanding virus-host interactions requires reverse-genetic studies using infectious cDNA clones.

Purpose of the Study:

To discuss the steps, rationales, and potential issues involved in cloning bee-infecting RNA viruses.
To provide insights from failed attempts at cloning specific viral isolates.
To facilitate the development of reverse-genetic studies for bee-infecting viruses.

Main Methods:

Review of established protocols for generating infectious cDNA clones of RNA viruses.
Discussion of challenges encountered in viral cloning, including isolate selection and genetic stability.
Case study detailing the cloning of a Deformed wing virus isolate.

Main Results:

Few infectious cDNA clones of bee-infecting viruses have been reported despite decades of research.
Cloning bee-infecting RNA viruses presents significant technical challenges.
Failed cloning attempts highlight the complexities of working with certain viral isolates.

Conclusions:

Infectious cDNA clones are essential tools for reverse-genetic studies of bee-infecting viruses.
Addressing the challenges in viral cloning will accelerate research into pollinator decline.
Further development of cloning techniques is needed to fully elucidate the role of viruses in pollinator health.