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Updated: Jul 1, 2025
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
Published on: March 13, 2018
Sensitive and rapid detection of Culex pipiens and Aedes albopictus
Xiao Wei1, Biao Meng1,2, Yan Li1
1Department of Pest Management, Centers for Disease Control and Prevention of People's Liberation Army (PLA), Beijing, China.
Background:
Culex pipiens and Aedes albopictus are closely related to human life, and transmit a variety of viruses, causing serious harm to human health. Cytochrome c oxidase I (COI) gene has been selected as a marker gene for studying phylogeny and molecular evolution of species and is also an effective molecular marker for studying the evolutionary mechanism and systematic reconstruction of diptera insects.
Methods:
A loop-mediated isothermal amplification (LAMP) method for the rapid and sensitive detection of Cx. pipiens and Ae. albopictus were first described in this study. The experimental results were verified by real-time PCR.
Results:
Our study showed the lower limit of sample concentration that can be detected by LAMP method is 0.5 pg/μl within 20 min for Cx. pipiens, and 1 pg/μl within 20 min for Ae. albopictus, which were more sensitive than PCR method. Validation tests with field samples showed LAMP method had good specificity and sensitivity and could identify the target species quickly and accurately.
Conclusion:
The LAMP method developed in this study allowed the rapid and sensitive detection of Cx. pipiens and Ae. albopictus, which will be expected to be used for mass screening in batches of the field.
