Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in Arabidopsis

Understanding the generation, movement, uptake, and perception of mobile defense signals is key for unraveling the systemic resistance programs in flowering plants against pathogens. Here, we present a protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid (AZA) in Arabidopsis thaliana. We describe steps to assess ¹⁴C-AZA uptake into leaf discs and its movement from local to systemic tissues. We also detail the assay for uptake and movement of ²H-AZA from roots to the shoot. For complete details on the use and execution of this protocol, please refer to Cecchini et al.^1,2.