Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Social evolution and diminished olfactory function in larval honey bees.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Privatized Health Care System in Times of Crisis: South Korea's Health Care System Response to the COVID-19 Pandemic.

International journal of social determinants of health and health services·2026
Same author

Electrochemical Strain-Release Difunctionalization of Azabicyclo-[1.1.0]butanes with Nitroarenes.

Journal of the American Chemical Society·2026
Same author

MilliMap: interactive closed-loop analysis for spatial omics.

bioRxiv : the preprint server for biology·2026
Same author

Systematic investigation of double emulsion dewetting dynamics for the robust production of giant unilamellar vesicles.

Lab on a chip·2026
Same author

RESCUE: recovery of unattributed expression patterns in spatial transcriptomics.

Nature communications·2026

Related Experiment Video

Updated: Jul 1, 2025

Production and Targeting of Monovalent Quantum Dots
10:16

Production and Targeting of Monovalent Quantum Dots

Published on: October 23, 2014

25.5K

Versatile Prepolymer Platform for Controlled Tailoring of Quantum Dot Surface Properties.

JuYeon Lee1,2, Giselle Soares1, Calvin Doty1

  • 1Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.

ACS Applied Materials & Interfaces
|March 12, 2024
PubMed
Summary

Researchers can now easily engineer quantum dots (QDs) for bioimaging using a new poly(pentafluorophenyl acrylate) (PPFPA) prepolymer. This platform reduces nonspecific binding and enables sensitive detection of intracellular proteins, overcoming key limitations in QD surface modification.

Keywords:
bioimagingcontrollabilityligandsprepolymerquantum dotssurface engineering

More Related Videos

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

18.2K
A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals
09:58

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

Published on: May 10, 2018

9.6K

Related Experiment Videos

Last Updated: Jul 1, 2025

Production and Targeting of Monovalent Quantum Dots
10:16

Production and Targeting of Monovalent Quantum Dots

Published on: October 23, 2014

25.5K
Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

18.2K
A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals
09:58

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

Published on: May 10, 2018

9.6K

Area of Science:

  • Materials Science
  • Biotechnology
  • Nanotechnology

Background:

  • Quantum dots (QDs) show great potential for bioimaging but face challenges in surface engineering.
  • Existing methods often require specialized synthetic expertise and can lead to unwanted side reactions.

Purpose of the Study:

  • To develop a user-friendly prepolymer platform for precise quantum dot surface engineering.
  • To create multidentate polymeric ligands that minimize hydrolysis and nonspecific binding.
  • To enable sensitive detection of intracellular targets using quantum dots.

Main Methods:

  • Synthesis of poly(pentafluorophenyl acrylate) (PPFPA) prepolymer using commercially available reagents.
  • Surface functionalization of quantum dots with PPFPA-derived ligands.
  • Live cell imaging to assess nonspecific binding.
  • Development of compact QD-F(ab')2 probes for immunofluorescence.

Main Results:

  • PPFPA prepolymer demonstrated minimal hydrolysis and reduced nonspecific binding in live cell imaging compared to conventional methods.
  • Biomolecule-conjugated QDs were synthesized, enabling sensitive detection of cytosolic proteins.
  • QD-based immunofluorescence achieved a ~50-fold higher signal-to-noise ratio for alpha-tubulin detection compared to organic dyes.

Conclusions:

  • PPFPA offers a versatile and accessible platform for quantum dot surface engineering.
  • This approach overcomes limitations in QD bioimaging, particularly for intracellular targets.
  • The developed platform facilitates the broader application of quantum dots in scientific research.