Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

8.0K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
8.0K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.4K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.4K
Computed Tomography01:10

Computed Tomography

4.5K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
4.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Extracellular matrix remodeling modifies structural responses to ventilator-induced lung injury: a multiscale correlative imaging study.

Respiratory research·2026
Same author

3D reconstruction of the nymphal feeding apparatus of Philaenus spumarius.

Micron (Oxford, England : 1993)·2026
Same author

Wave-optics simulation model for full-field modulation-based tensor tomography using a random wavefront modulator.

Optics express·2026
Same author

A coincidence-based response matrix for correction of charge sharing spectral distortions in photon counting detectors.

Medical physics·2026
Same author

Probing Electrode Heterogeneity with 2D X‑ray Absorption Imaging.

The journal of physical chemistry. C, Nanomaterials and interfaces·2026
Same author

DPP3 deficiency impacts bone quality and sensitizes to metabolic alterations.

Bone·2026

Related Experiment Video

Updated: Jul 1, 2025

Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue
06:28

Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue

Published on: October 7, 2014

13.3K

Edge-illumination spectral phase-contrast tomography.

Luca Brombal1,2, Fulvia Arfelli1,2, Francesco Brun2,3

  • 1Department of Physics, University of Trieste, Via A. Valerio 2, I-34127 Trieste, Italy.

Physics in Medicine and Biology
|March 12, 2024
PubMed
Summary

This study introduces spectral phase-contrast computed tomography (CT), merging spectral and phase-contrast imaging for enhanced material decomposition. The technique achieves high accuracy in mapping water, calcium, and iodine, improving signal-to-noise ratio.

Keywords:
edge illuminationphoton-counting detectorspectral imagingx-ray phase-contrast imagingx-ray tomography

More Related Videos

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research
15:18

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Published on: January 12, 2013

16.4K
Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

3.3K

Related Experiment Videos

Last Updated: Jul 1, 2025

Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue
06:28

Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue

Published on: October 7, 2014

13.3K
Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research
15:18

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Published on: January 12, 2013

16.4K
Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

3.3K

Area of Science:

  • Medical Imaging
  • Physics
  • Materials Science

Background:

  • X-ray spectral and phase-contrast imaging have advanced independently.
  • Combining these techniques offers potential for enhanced material characterization.

Purpose of the Study:

  • To demonstrate the first combination of spectral and phase-contrast computed tomography (CT).
  • To develop and validate a theoretical model for spectral phase-contrast material decomposition.
  • To assess the accuracy and sensitivity of the combined technique.

Main Methods:

  • Utilized edge-illumination technique with a CdTe small-pixel spectral detector.
  • Developed a theoretical model from spectral decomposition to material decomposition.
  • Quantified accuracy and sensitivity using experimental data from phantoms and a murine model.

Main Results:

  • Achieved simultaneous mass density maps of water, calcium, and iodine with high accuracy (1.1%, 3.5%, 1.9% RMSE).
  • Reported a 9-fold increase in signal-to-noise ratio for the water channel compared to standard spectral decomposition.
  • Demonstrated 3D visualization of soft tissue, bone, and vasculature in an iodine-perfused murine model.

Conclusions:

  • Spectral phase-contrast CT combines high-Z material discrimination with soft tissue sensitivity.
  • The technique shows promise for advanced medical imaging applications, including micro CT.
  • The experimental setup is adaptable for both synchrotron and conventional laboratory X-ray systems.