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Leptospires colonial variations.

E M Petrov, Y G Chernukha

    Zentralblatt Fur Bakteriologie, Parasitenkunde, Infektionskrankheiten Und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie Und Parasitologie
    |June 1, 1979
    PubMed
    Summary

    Colonial variants of certain bacterial serovars showed differences in virulence and cell morphology, despite maintaining consistent antigenic properties. These variations arose spontaneously, highlighting potential evolutionary pathways in bacterial populations.

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    Area of Science:

    • Microbiology
    • Bacteriology
    • Pathogen Evolution

    Background:

    • Bacterial serovars can exhibit heterogeneity in colonial morphology.
    • Understanding this heterogeneity is crucial for characterizing pathogen behavior and evolution.

    Purpose of the Study:

    • To investigate the relationship between colonial morphology, antigenic properties, virulence, and cell morphology in bacterial serovars.
    • To determine the frequency of spontaneous mutations affecting colonial morphology.

    Main Methods:

    • Isolation and characterization of bacterial strains representing serovars mozdok, monjakov, kazakhstanica I, and patoc.
    • Colony recloning and analysis of antigenic properties using microagglutination and agglutinin absorption tests.
    • Virulence (LD50) and renal infection (ID50) assays in hamsters.
    • Microscopic examination of cell morphology.
    • Mutation frequency determination in a solid Tween-80 albumin medium.

    Main Results:

    • Six of 12 strains displayed heterogeneous colonial morphology.
    • Recloned colonial variants retained identical antigenic properties.
    • Distinct differences in virulence (LD50) and renal infection (ID50) were observed between colonial variants of serovar mozdok.
    • Cell morphology differences (hooked vs. straight) were noted in some variants.
    • Spontaneous colonial mutants appeared at a frequency of 10^-9 to 10^-8 per bacterium per generation, without altered antigenic properties, virulence, or cell morphology.

    Conclusions:

    • Colonial morphology variations within bacterial serovars can be uncoupled from antigenic properties.
    • These morphological changes can correlate with significant differences in virulence and host-pathogen interactions.
    • Spontaneous mutation is a mechanism for generating colonial diversity in bacteria.

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