Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Towards the construction of a virtual yeast.

Nature·2026

Same author

Analysis of Fall and Jump Behaviors in Freely Moving <i>Drosophila melanogaster</i> Using 58 fps Video.

Insects·2026

Same author

Predictive capacity of mismatch repair status in the use of immune checkpoint inhibitors for the treatment of aggressive pituitary tumors and pituitary carcinomas: An illustrative case report and literature review.

Surgical neurology international·2026

Same author

Population-scale chemical response revealed by a barcoded yeast collection.

Nature communications·2026

Same author

The 2025 Westlake Autumn Symposium for Al Proteomics and Virtual Cell.

Genomics, proteomics & bioinformatics·2026

Same author

Hepatotoxicity of External Radiotherapy for Hepatocellular Carcinoma in the Setting of Prior Yttrium-90 Radioembolization.

American journal of clinical oncology·2026

Same journal

Paradoxical non-catalytic kinase functions are driven by inhibitor-induced displacement of autoinhibitory domains.

Molecular systems biology·2026

Same journal

E. coli prepares for starvation by dramatically remodeling its proteome in the first hours after loss of nutrients.

Molecular systems biology·2026

Same journal

Common xenobiotics modulate gut microbial responses to low‑calorie sweeteners in vitro.

Molecular systems biology·2026

Same journal

ParTIpy: a scalable framework for archetypal analysis and Pareto task inference.

Molecular systems biology·2026

Same journal

Quantitative interactome mapping of skeletal muscle insulin resistance.

Molecular systems biology·2026

Same journal

Interpretable multi-omics integration across mixed-order tensors with MANTRA.

Molecular systems biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 1, 2025

Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy

Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy

Published on: November 20, 2018

PIFiA: self-supervised approach for protein functional annotation from single-cell imaging data.

Anastasia Razdaibiedina^1,2,3, Alexander Brechalov^1,2, Helena Friesen²

¹Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.

Molecular Systems Biology

|March 13, 2024

Summary

This summary is machine-generated.

We developed PIFiA, a self-supervised method for protein functional annotation using fluorescence microscopy images. PIFiA extracts robust features from cell images, enabling new insights into protein organization and function.

Keywords:

Imaging Machine Learning Protein Self-supervised Single-cell

More Related Videos

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Related Experiment Videos

Last Updated: Jul 1, 2025

Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy

Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy

Published on: November 20, 2018

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Area of Science:

Cell biology
Proteomics
Bioimaging

Background:

Fluorescence microscopy provides single-cell resolution of protein localization.
Extracting functional information from cell images is challenging.
Existing methods often require supervised labels and struggle with noise.

Purpose of the Study:

To develop a self-supervised approach for protein functional annotation from microscopy data.
To generate robust and noise-invariant feature profiles for proteins.
To enable quantitative analysis of protein organization and function.

Main Methods:

Developed PIFiA (Protein Image-based Functional Annotation), a self-supervised learning method.
Applied PIFiA to the global yeast ORF-GFP collection.
Generated protein feature profiles from single-cell images.

Main Results:

PIFiA outperforms existing methods in molecular representation learning.
Generated hierarchical functional organization, studied cell heterogeneity, and identified functional modules.
Confirmed new PIFiA predictions, suggesting novel biological roles for proteins.

Conclusions:

PIFiA is an effective self-supervised tool for protein functional annotation.
The method facilitates the exploration of protein organization and cellular heterogeneity.
PIFiA provides a valuable resource for quantitative cell biology research.