Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

6.5K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.5K
Abnormal Proliferation02:23

Abnormal Proliferation

4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

6.3K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.3K
Negative Regulator Molecules01:23

Negative Regulator Molecules

35.4K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The difference in scotopic and photopic pupil responses: a potential indicator for long-term glycemic management.

Frontiers in neurology·2026
Same author

An Adams' Arch -based anatomical classification system guiding internal fixation strategies for non-displaced femoral neck fractures in elderly patients: a finite element analysis.

BMC musculoskeletal disorders·2026
Same author

Translating policy into practice: teacher agency amid cognitive and ecological constraints in enacting competency-based assessment.

Frontiers in psychology·2026
Same author

Co-exposure to environmental lead and hypertension exacerbates anxiety and depression via mtDNA-mediated cGAS phase separation.

Environmental pollution (Barking, Essex : 1987)·2026
Same author

Efficacy and safety of combined immune therapy for advanced cervical cancer: a systematic review and meta-analysis.

Frontiers in oncology·2026
Same author

Plane-wave-like laser ultrasonic inspection of multilayer delaminations using beam-expanded excitation and migration-based reconstruction.

Ultrasonics·2026

Related Experiment Video

Updated: Jul 1, 2025

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

6.9K

Linker Histone H1.4 Inhibits the Growth, Migration and EMT Process of Non-Small Cell Lung Cancer by Regulating ERK1/2

Qian Chen1, Mengqi Yang1, Xinyue Duan1

  • 1School of Life Sciences, Anhui University, Hefei, Anhui Province, 230601, PR China.

Biochemical Genetics
|March 13, 2024
PubMed
Summary

Histone H1.4 suppresses non-small cell lung cancer (NSCLC) progression by inhibiting ERK1/2 signaling. This pathway is crucial for NSCLC cell viability, migration, invasion, and epithelial-mesenchymal transition, offering a potential therapeutic target.

Keywords:
EMTERK1/2Linker Histone H1.4MigrationNSCLC

More Related Videos

An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells
08:54

An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells

Published on: July 20, 2014

14.1K
Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics
06:00

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics

Published on: May 14, 2016

11.0K

Related Experiment Videos

Last Updated: Jul 1, 2025

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

6.9K
An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells
08:54

An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells

Published on: July 20, 2014

14.1K
Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics
06:00

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics

Published on: May 14, 2016

11.0K

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Histone H1.4 is implicated in tumorigenesis, but its role in non-small cell lung cancer (NSCLC) remains undefined.
  • Understanding histone variants' functions is critical for cancer research.

Purpose of the Study:

  • To investigate the role and mechanism of histone H1.4 in non-small cell lung cancer (NSCLC).
  • To explore the potential of the H1.4-ERK pathway as a therapeutic target for NSCLC.

Main Methods:

  • Utilized shRNA knockdown and overexpression of H1.4 in NSCLC cell lines (A549, H1299).
  • Assessed cell viability, migration, invasion, and epithelial-mesenchymal transition (EMT).
  • Analyzed ERK1/2 expression and phosphorylation; performed chromatin immunoprecipitation to assess H1.4 binding to the ERK1/2 promoter.

Main Results:

  • H1.4 overexpression inhibited NSCLC cell viability, migration, invasion, and EMT.
  • H1.4 knockdown promoted these processes.
  • H1.4 suppressed ERK1/2 expression transcriptionally and reduced its phosphorylation.
  • ERK re-expression reversed H1.4's inhibitory effects on NSCLC progression.

Conclusions:

  • The H1.4-ERK pathway is a key regulator of NSCLC cell viability, migration, invasion, and EMT.
  • H1.4 acts as a transcriptional suppressor of ERK1/2 in NSCLC.
  • Targeting the H1.4-ERK pathway presents a promising therapeutic strategy for NSCLC.