Related Experiment Video

Updated: Jul 1, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.6K

Variant Analysis of miRNA Regulatory Genes in 35 Sporadic Lung Carcinoma Tumors

Özkan Bağcı1, Ebru Marzioğlu Özdemir2, Batuhan Şanlıtürk2

  • 1Department of Medical Genetics, Selcuk University, School of Medicine, Konya, Turkey. ozkan.bagci@selcuk.edu.tr.

Doklady. Biochemistry and Biophysics
|March 13, 2024
PubMed

Insights

Genomic changes in miRNA biogenesis genes may explain varied responses to lung cancer therapies and metastasis. These findings highlight potential new biomarkers for predicting patient outcomes and guiding treatment strategies.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Lung cancer has high global mortality.
  • EGFR, BRAF, and KRAS genes are key in lung cancer, targeted by therapies.
  • Patient responses to Tyrosine Kinase Inhibitors (TKIs) vary, suggesting a need for new biomarkers and investigation into epigenetic factors.

Purpose of the Study:

  • To investigate genetic variations in miRNA biogenesis genes (AGO2, DICER, DROSHA) and key cancer genes (EGFR, KRAS, BRAF) in sporadic lung cancer.
  • To identify potential novel biomarkers for predicting patient response to therapy and metastasis.

Main Methods:

  • Sequence analysis of AGO2, DICER, DROSHA, EGFR, KRAS, and BRAF genes.
  • Study included 35 patients with sporadic lung cancer.

Main Results:

  • Novel variations in miRNA biogenesis genes were identified.
  • Four known EGFR variants were found.
  • A significant association (p:0.02) was observed between mutations in miRNA biogenesis genes and metastasis.

Conclusions:

  • Genomic alterations in miRNA biogenesis genes could contribute to differential patient responses to therapies.
  • These dysregulations may play a role in metastasis, particularly in EGFR wild-type tumors.
  • MiRNA biogenesis gene variations represent potential prognostic biomarkers in lung cancer.
Keywords:
AGO2DICERDROSHAEGFRlung cancermiRNA biogenesis

More Related Videos

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

8.4K
CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

2.4K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K