Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessment of out-of-field doses in pediatric radiotherapy.

Radiological physics and technology·2026
Same author

Evaluation of Cone-Beam Computed Tomography imaging protocols in radiation therapy: Dose assessment in pediatric and adult phantoms and a national survey of clinical practice.

Journal of medical imaging and radiation sciences·2026
Same author

Cork By-Products as Bioactive Ingredients: From Waste Valorization to Pharmaceutical Prototypes.

Molecules (Basel, Switzerland)·2026
Same author

Sterilization by Ozone: Effects on Electrospun Polycaprolactone Membrane Properties and Cell Viability.

International journal of biomaterials·2026
Same author

Moderately hypofractionated partial breast reirradiation: early clinical results and dosimetric considerations in the context of 2<sup>nd</sup> (partial) breast irradiation.

Reports of practical oncology and radiotherapy : journal of Greatpoland Cancer Center in Poznan and Polish Society of Radiation Oncology·2025
Same author

Chitosan/Alginate Polyelectrolyte Magnetic Gel Nanoarchitectonics with Tunable Mechanical Properties for Magnetic Hyperthermia and Sustained Release of 5-Fluorouracil.

ACS applied materials & interfaces·2025

Related Experiment Video

Updated: Jul 1, 2025

miRNA Expression Analyses in Prostate Cancer Clinical Tissues
11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

10.9K

Radiotherapy Metastatic Prostate Cancer Cell Lines Treated with Gold Nanorods Modulate miRNA Signatures.

Sílvia Soares1,2,3,4,5,6,7, Fátima Aires7, Armanda Monteiro7

  • 1(i3S), Instituto de Investigação e Inovação em Saúde, 4200-135 Porto, Portugal.

International Journal of Molecular Sciences
|March 13, 2024
PubMed
Summary

Modulating microRNA (miRNA) with gold nanorods and radiotherapy shows promise for prostate cancer (PCa) treatment. This approach may personalize PCa therapy by targeting specific miRNA signatures in metastatic cells.

Keywords:
gold nanorodsmicroRNAsprostate cancer cell linesradiotherapy

More Related Videos

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR
08:30

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

Published on: May 16, 2012

24.5K
Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases
09:48

Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases

Published on: August 23, 2024

362

Related Experiment Videos

Last Updated: Jul 1, 2025

miRNA Expression Analyses in Prostate Cancer Clinical Tissues
11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

10.9K
MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR
08:30

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

Published on: May 16, 2012

24.5K
Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases
09:48

Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases

Published on: August 23, 2024

362

Area of Science:

  • Oncology
  • Nanomedicine
  • Molecular Biology

Background:

  • MicroRNA (miRNA) modulation is a potential strategy to enhance prostate cancer (PCa) response to radiotherapy (RT).
  • Pegylated gold nanorods (AuNPr-PEG) are explored for PCa treatment and miRNA delivery.
  • Understanding miRNA signature changes is crucial for optimizing PCa radiosensitivity.

Purpose of the Study:

  • To evaluate the effect of hypofractionated RT and AuNPr-PEG on the miRNA signature of PCa cells.
  • To investigate the potential of AuNPr-PEG and RT in modulating specific miRNAs (miRNA-95, miRNA-106-5p, miRNA-145-5p, miRNA-541-3p).
  • To explore personalized treatment strategies for PCa based on miRNA expression.

Main Methods:

  • Utilized RT-qPCR to analyze miRNA expression levels.
  • Tested three human metastatic prostate cell lines (PC3, DU145, LNCaP) and one non-tumor cell line (HprEpiC).
  • Applied hypofractionated RT (2.5 Gy/day for 3 days) with and without AuNPr-PEG pre-treatment.

Main Results:

  • MiRNA expression varied significantly based on cell type and treatment combination (RT + AuNPr-PEG).
  • Pre-treatment with AuNPr-PEG followed by hypofractionated RT decreased the expression of miRNA-95, miRNA-106, miRNA-145, and miRNA-541-3p.
  • The study identified specific miRNA expression patterns influenced by the combined treatment.

Conclusions:

  • Hypofractionated RT combined with AuNPr-PEG can modulate the miRNA signature in PCa cells.
  • AuNPr-PEG may enhance metastatic cell radiosensitivity, suggesting a role in improving RT efficacy.
  • Personalized PCa treatment could be developed based on individual miRNA signatures and response to AuNPr-PEG and RT.