Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Prognostic Value Of Circulating Cell-free Dna Concentration And Neutrophil-to-lymphocyte Ratio In Patients With Pancreatic Ductal Adenocarcinoma: A Prospective Cohort Study

Prognostic Value of Circulating Cell-Free DNA Concentration and Neutrophil-to-Lymphocyte Ratio in Patients with Pancreatic Ductal Adenocarcinoma: A Prospective Cohort Study

Bianca Varzaru1,2, Razvan Andrei Iacob1,2,3, Stefania Bunduc1,2,3

  • 1Faculty of Medicine, Carol Davila University of Medicine and Pharmacy, 020021 Bucharest, Romania.

International Journal of Molecular Sciences
|March 13, 2024

Related Experiment Videos

Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma
05:44

Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma

Published on: November 5, 2020

4.3K
Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence
10:29

Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence

Published on: August 14, 2019

10.6K
Ultrasound-Guided Orthotopic Implantation of Murine Pancreatic Ductal Adenocarcinoma
06:21

Ultrasound-Guided Orthotopic Implantation of Murine Pancreatic Ductal Adenocarcinoma

Published on: November 19, 2019

11.2K

View abstract on PubMed

Summary
This summary is machine-generated.

Circulating cell-free DNA (ccfDNA) and neutrophil-to-lymphocyte ratio (NLR) predict survival in pancreatic cancer. Higher ccfDNA and NLR levels are associated with shorter overall survival in patients with pancreatic ductal adenocarcinoma (PDAC).

Area of Science:

  • Oncology
  • Molecular Diagnostics

Background:

  • Circulating cell-free DNA (ccfDNA) quantity is linked to prognosis in various cancers.
  • The prognostic value of ccfDNA and neutrophil-to-lymphocyte ratio (NLR) in pancreatic ductal adenocarcinoma (PDAC) requires further investigation.

Purpose of the Study:

  • To evaluate the prognostic significance of ccfDNA levels and NLR in patients with PDAC.
  • To determine if the combination of ccfDNA and NLR improves prognostic accuracy for PDAC.

Main Methods:

  • Peripheral blood samples were collected from 82 PDAC patients before treatment.
  • Plasma DNA was isolated to determine ccfDNA concentration.
  • Neutrophil-to-lymphocyte ratio (NLR) was calculated.

Main Results:

  • ccfDNA levels correlated with age and tumor burden.
Keywords:
cell-free DNAneutrophil-to-lymphocyte ratiooverall survivalpancreatic ductal adenocarcinoma

Related Experiment Videos

Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma
05:44

Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma

Published on: November 5, 2020

4.3K
Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence
10:29

Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence

Published on: August 14, 2019

10.6K
Ultrasound-Guided Orthotopic Implantation of Murine Pancreatic Ductal Adenocarcinoma
06:21

Ultrasound-Guided Orthotopic Implantation of Murine Pancreatic Ductal Adenocarcinoma

Published on: November 19, 2019

11.2K
  • Elevated NLR (≥3.31) was associated with significantly shorter overall survival (OS) (4 vs. 10 months; p=0.011).
  • Higher ccfDNA concentration (≥25.79 ng/mL) was strongly linked to shorter OS (4 vs. 8 months; p=0.009).
  • Baseline ccfDNA was an independent prognostic factor for OS (HR 0.45, p=0.041).
  • Combining ccfDNA and NLR enhanced prognostic accuracy for PDAC patients.

    • Conclusions:

    • ccfDNA concentration and NLR are independent predictors of survival in PDAC.
    • The combination of ccfDNA and NLR shows potential for improved prognostic assessment in PDAC.
    • Further studies are warranted to validate this combined marker for guiding therapeutic decisions.