Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Non-Destructive Determination of Moisture Content in Husk-On Fresh Corn Using Multichannel Visible-Near-Infrared Spectroscopy Combined with Deep Learning.

Applied spectroscopy·2026
Same author

Deep learning regression model based on data pairing and pseudo-label fusion for NIR predictive modeling in food and pharmaceutical quality analysis.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same author

TTI and pH-responsive dual colorimetric sensor arrays combined with a cascaded deep learning approach for dynamic monitoring of freshness of fresh-cut fruits.

Food chemistry·2025
Same author

COS-DeformDeep: Adaptive 2T2D spectral feature extraction method for improving the component identification performance in mixtures based on handheld Raman technology.

Analytica chimica acta·2025
Same author

A feature-enhanced approach based on joint domain alignment and multi-order derivative spectral reconstruction for predicting apple firmness using Vis-NIR spectroscopy.

Food chemistry·2025
Same author

An intelligent fruit freshness monitoring system using hydrophobic indicator labels based on methylcellulose, k-carrageenan, and sodium tripolyphosphate, combined with deep learning.

International journal of biological macromolecules·2025

Related Experiment Video

Updated: Jul 8, 2026

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

22.2K

Developing a Portable Fluorescence Imaging Device for Fish Freshness Detection.

Zheng Pan1, Min Huang1, Qibing Zhu1

  • 1Key Laboratory of Advanced Process Control for Light Industry, Ministry of Education, Jiangnan University, Wuxi 214122, China.

Sensors (Basel, Switzerland)
|March 13, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces a low-cost, portable fluorescence imaging device for rapid fish freshness detection. The system achieves high accuracy (97.10%) and fast results (under 1 second), improving aquatic product safety.

Keywords:
FPGAYOLOv4-tinyfish freshness detectionfluorescence imaginglow-costrapid

More Related Videos

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone
06:42

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

Published on: April 11, 2013

23.7K
Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
08:27

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

9.1K

Related Experiment Videos

Last Updated: Jul 8, 2026

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

22.2K
Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone
06:42

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

Published on: April 11, 2013

23.7K
Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
08:27

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

9.1K

Area of Science:

  • * Food Science and Technology
  • * Applied Physics and Engineering

Background:

  • * Ensuring aquatic product safety necessitates rapid fish freshness detection.
  • * Existing optical methods face challenges in efficiency, cost, and equipment integration.
  • * Limitations of current fish freshness detection technologies hinder widespread adoption.

Purpose of the Study:

  • * To develop a low-cost, portable fluorescence imaging device for rapid fish freshness assessment.
  • * To overcome the limitations of current fish freshness detection methods.
  • * To provide an accessible tool for real-time fish quality evaluation.

Main Methods:

  • * Development of a portable fluorescence imaging device utilizing ultraviolet-light-emitting diode (UV-LED) excitation.
  • * Integration of a Field-Programmable Gate Array (FPGA) board (ZYNQ XC7Z020) for master control.
  • * Application of the YOLOv4-Tiny model embedded in FPGA for fluorescence image processing and freshness determination.

Main Results:

  • * The developed device achieved an average fish freshness detection accuracy of 97.10% using grass carp fillets.
  • * Demonstrated real-time performance with a detection time below 1 second per sample.
  • * Exhibited low power consumption (47.1 W total), indicating efficient operation.

Conclusions:

  • * The developed low-cost, portable fluorescence imaging device offers a rapid and accurate solution for fish freshness evaluation.
  • * This technology has the potential to significantly enhance aquatic product safety and quality control.
  • * The system's efficiency, low cost, and portability make it suitable for practical applications in the food industry.