Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K
Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

1.7K
When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
1.7K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

4.7K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
4.7K
IR Spectrometers01:25

IR Spectrometers

1.1K
There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
1.1K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

5.7K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
5.7K
IR Frequency Region: Fingerprint Region01:03

IR Frequency Region: Fingerprint Region

880
IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the...
880

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predicting human mRNA isoform levels from site-specific splicing kinetics <i>in silico</i>.

bioRxiv : the preprint server for biology·2026
Same author

Label-free multimodal nonlinear microscopy enabled by an optical parametric generator.

APL photonics·2026
Same author

Transfer-Matrix Framework for Modeling Mid-Infrared Vibrational Circular Dichroism Spectra.

Analytical chemistry·2026
Same author

Label-free correlative morpho-chemical tomography of 3D kidney mesangial cells.

Journal of biomedical optics·2026
Same author

Quantum Cascade Laser-Based Vibrational Circular Dichroism Imaging for Chiral Biosensing.

Annual review of analytical chemistry (Palo Alto, Calif.)·2026
Same author

Time-Domain Quantum Cascade Laser-Based Vibrational Circular Dichroism Spectroscopy with Linear Dichroism Monitoring.

Analytical chemistry·2025
Same journal

Methanol Partial Oxidation on Cu(111) and PtCu(111) Single-Atom Alloy Surfaces: Effect of Surface Oxygen Coverage on Selectivity.

The journal of physical chemistry. C, Nanomaterials and interfaces·2026
Same journal

Yb<sup>3+</sup>-Doped GaN Nanoceramics as a New Material for Broad Band White Light Emission.

The journal of physical chemistry. C, Nanomaterials and interfaces·2026
Same journal

Energetic and Structural Insights into Water Confined in Hydrophobic Nanopores.

The journal of physical chemistry. C, Nanomaterials and interfaces·2026
Same journal

Impact of Morphology and Composition of Graphene Aerosol-Gel Particles in Thin Films on Ultrafast Carrier Dynamics Studied via Transient Absorption Spectroscopy.

The journal of physical chemistry. C, Nanomaterials and interfaces·2026
Same journal

Rapid Determination of SiO<sub>2</sub> Shell Thickness on Au Core Nanoparticles via Differential Centrifugal Sedimentation for SHINERS.

The journal of physical chemistry. C, Nanomaterials and interfaces·2026
Same journal

Effect of Exchange-Correlation Functionals on Schottky Barriers at Si/Metal Interfaces.

The journal of physical chemistry. C, Nanomaterials and interfaces·2026
See all related articles

Related Experiment Video

Updated: Jul 1, 2025

Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing
10:42

Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing

Published on: March 22, 2019

6.2K

Resolution Limit in Infrared Chemical Imaging.

Yamuna Phal1, Luke Pfister2, P Scott Carney3

  • 1Department of Electrical and Computer Engineering, University of Illinois at Urbana - Champaign, Urbana, Illinois 61801, United States; Beckman Institute for Advanced Science and Technology, Urbana, Illinois 61801, United States.

The Journal of Physical Chemistry. C, Nanomaterials and Interfaces
|March 13, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces an information theory approach to measure how well spectral data in chemical imaging can pinpoint spatial locations. It helps define resolution limits by considering signal quality and spectral differences in mid-infrared imaging.

More Related Videos

Super-resolution Imaging of the Bacterial Division Machinery
08:47

Super-resolution Imaging of the Bacterial Division Machinery

Published on: January 21, 2013

11.8K
Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

3.9K

Related Experiment Videos

Last Updated: Jul 1, 2025

Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing
10:42

Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing

Published on: March 22, 2019

6.2K
Super-resolution Imaging of the Bacterial Division Machinery
08:47

Super-resolution Imaging of the Bacterial Division Machinery

Published on: January 21, 2013

11.8K
Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

3.9K

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Microscopy

Background:

  • Chemical imaging integrates optical microscopy and vibrational spectroscopy for spatial and spectral analysis.
  • Mid-infrared (IR) absorption imaging offers high-quality spectra with microscopic spatial resolution.
  • Understanding the limits of spatial and spectral object resolution in IR chemical imaging is crucial but not fully explored.

Purpose of the Study:

  • To develop an information theory-based method for quantifying spatial localization capabilities using spectral data in chemical imaging.
  • To investigate the influence of spectral information on spatial sensitivity, an under-explored aspect of IR spectroscopic imaging.
  • To derive resolution limits in IR spectroscopic imaging by considering signal-to-noise ratio and spectral separation.

Main Methods:

  • Utilized an information theory framework to quantify spatial localization.
  • Explicitly considered the joint effects of signal-to-noise ratio (SNR) and spectral separation.
  • Derived resolution limits applicable to experimental settings in IR spectroscopic imaging.

Main Results:

  • An information theory-based approach was successfully developed to quantify spatial localization in chemical imaging.
  • The study highlights the significance of spectral information, SNR, and spectral separation in determining spatial resolution limits.
  • Established a quantitative method to assess the spatial localization capability of spectral data.

Conclusions:

  • The developed approach provides a novel way to understand and quantify the spatial resolution limits in IR spectroscopic imaging.
  • This work addresses the under-explored influence of spectral information on spatial sensitivity.
  • The findings are significant for optimizing optical design and experimental parameters in chemical imaging.