Exploring the diversity, bioactivity of endophytes, and metabolome in Synsepalum dulcificum
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View abstract on PubMed
Summary
This summary is machine-generated.Endophytes from the miracle berry plant (Synsepalum dulcificum) show high diversity and potential for producing bioactive compounds. Many isolates demonstrated antimicrobial, antioxidant, and alpha-glucosidase inhibitory activities, suggesting applications in drug development.
Area Of Science
- Microbiology and Biotechnology
- Natural Product Chemistry
- Pharmacology
Background
- Synsepalum dulcificum, known for its edible and medicinal properties, has unexplored endophytic microbial resources.
- Endophytes are microorganisms residing within plant tissues, often producing bioactive secondary metabolites.
- Understanding endophyte diversity and function in S. dulcificum can reveal novel sources of therapeutic compounds.
Purpose Of The Study
- To investigate the microbial diversity (fungal and bacterial) of Synsepalum dulcificum.
- To analyze the correlation between endophytic communities and plant metabolites.
- To evaluate the biological activities (antimicrobial, antioxidant, alpha-glucosidase inhibition) of isolated endophytes.
Main Methods
- High-throughput sequencing (ITS and 16S rDNA) for endophytic fungal and bacterial identification.
- Metabolomic analysis of S. dulcificum tissues.
- Phylogenetic analysis of isolated strains.
- Culturing of endophytes and evaluation of antimicrobial, antioxidant, and alpha-glucosidase inhibitory activities using standard assays.
Main Results
- Identification of 4,913 fungal and 1,703 bacterial amplicon sequence variants (ASVs) across various plant tissues.
- Significant correlations observed between endophyte community composition and plant-derived metabolites.
- 88.6% of isolates showed antimicrobial activity, 55.7% antioxidant activity, and 85% of fungi exhibited alpha-glucosidase inhibitory activity.
Conclusions
- Synsepalum dulcificum harbors a highly diverse endophytic microbiome.
- These endophytes possess significant potential for producing bioactive metabolites with antimicrobial, antioxidant, and hypoglycemic properties.
- The findings provide a foundation for developing novel antibiotics, antioxidants, and hypoglycemic agents from S. dulcificum endophytes.
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