When will we have a clone? An industry perspective on the typical CLD timeline
Howard Clarke1, Anke Mayer-Bartschmid2, Chenxing Zheng3
1Seagen Inc., Cell Line Development, Bothell, Washington, USA.
Biotechnology Progress
|March 13, 2024
Summary
This study surveyed industry experts on cell line development (CLD) for biologics. It details the time and effort involved in antibody and complex molecule CLD processes, offering valuable insights for drug development.
Area of Science:
- Biopharmaceutical Development
- Molecular Biology
- Biotechnology
Background:
- Cell line development (CLD) is a critical but complex stage in biological drug manufacturing.
- Industry-wide understanding of CLD timelines and resource allocation is often limited.
Purpose of the Study:
- To survey and analyze the activities and effort involved in typical cell line development campaigns.
- To provide an industry perspective on the time and resources required for CLD, focusing on antibody and complex molecule development.
Main Methods:
- Surveys were distributed to BioPhorum members involved in the CLD working group.
- Data was collected on three key stages: Pre-transfection, Transfection, and Single Cell Cloning/Lead Clone Selection.
- Responses from an average of 27 members across different companies were analyzed.
Main Results:
- The survey covered 341 questions, distinguishing between antibody and complex molecule CLD processes.
- Detailed insights into the typical time and effort for each stage of CLD were interpreted.
- Challenges specific to complex molecule development were contrasted with antibody development.
Conclusions:
- The study offers a comprehensive industry view of the resources needed for CHO cell line development.
- Findings can help optimize CLD strategies and resource planning in biopharmaceutical companies.
- This work highlights the significant effort and structured approach required for successful CLD.
More Related Videos
Related Concept Videos
01:21Lineage Commitment
3.0K
Commitment is the process whereby stem cells:
3.0K
01:11High-Performance Liquid Chromatography: Introduction
2.0K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
In HPLC, two phases play a critical role in the separation process:
2.0K
02:20Longitudinal Research
12.0K
Sometimes we want to see how people change over time, as in studies of human development and lifespan. When we test the same group of individuals repeatedly over an extended period of time, we are conducting longitudinal research. Longitudinal research is a research design in which data-gathering is administered repeatedly over an extended period of time. For example, we may survey a group of individuals about their dietary habits at age 20, retest them a decade later at age 30, and then again...
12.0K
01:12Bewley Lattice Diagram
635
The Bewley lattice diagram, developed by L. V. Bewley, effectively organizes the reflections occurring during transmission-line transients. It visually represents how voltage waves propagate and reflect within a transmission line, making it easier to understand the complex interactions that occur.
635
01:16Confocal Fluorescence Microscopy
13.2K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.2K
01:28Load-frequency control
162
Load-frequency control (LFC) is vital for maintaining power system stability, ensuring that frequency and power flows remain within acceptable limits during load changes. Turbine-governor control eliminates rotor accelerations and decelerations following load changes. However, a steady-state frequency error persists when the change in the turbine-governor reference setting is zero. In an interconnected power system, each area agrees to export or import a scheduled amount of power through...
162