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An Additive Manufacturing Technique for the Facile and Rapid Fabrication of Hydrogel-based Micromachines with Magnetically Responsive Components
Published on: July 18, 2018
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Manufacturing of a Human Adipose-Derived Hydrogel
Jordan Robinson1, Haley Lassiter1, Katie Hamel1
1Obatala Sciences, Inc, New Orleans, LA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|March 13, 2024
Summary
Human decellularized adipose tissue (hDAT) hydrogels offer a promising in vitro model. Derived from abundant adipose tissue, hDAT hydrogels mimic native tissue for advanced tissue engineering applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Cell Biology
Background:
- Hydrogels are excellent in vitro models, surpassing traditional monolayer cultures.
- Natural hydrogels, particularly decellularized tissue-derived ones, are valuable for tissue engineering due to their native composition and architecture.
- Adipose tissue is an abundant and accessible source for creating such hydrogels.
Purpose of the Study:
- To highlight the potential of human decellularized adipose tissue (hDAT) hydrogels as an in vitro model.
- To present a manufacturing protocol for hDAT hydrogels.
Main Methods:
- Decellularization of human adipose tissue.
- Mechanical disruption of tissue.
- Chemical treatment using N-Lauroylsarcosine.
- Enzymatic digestion with pepsin and hydrochloric acid.
Main Results:
- Established a reproducible manufacturing protocol for hDAT hydrogels.
- hDAT hydrogels possess characteristics suitable for in vitro studies and tissue engineering.
- The protocol utilizes readily available adipose tissue resources.
Conclusions:
- hDAT hydrogels represent a clinically relevant and scalable biomaterial for tissue engineering.
- The developed protocol enables the production of a versatile hydrogel from adipose tissue.
- These hydrogels support cell functions essential for tissue regeneration.
