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Large-Scale, Automated Production of Adipose-Derived Stem Cell Spheroids for 3D Bioprinting
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Spheroid Formation of Human Adipose-Derived Stem Cells Using a Liquid Overlay Technique
Sara Al-Ghadban1, Bruce A Bunnell2
1Department of Microbiology, Immunology and Genetics, University of North Texas Health Science Center, Fort Worth, TX, USA.
Methods in Molecular Biology (Clifton, N.J.)
|March 13, 2024
Abstract
None:
Compared to two-dimensional monolayer culture, cells cultured in three-dimensional (3D) platforms provide a more biochemically and physiologically relevant environment to study cell-cell and cell-extracellular matrix interactions in vitro. Using the liquid overlay technique, a scaffold-free method to generate 3D spheroids from human adipose-derived stem cells is described.
