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Related Experiment Video

Updated: Jul 1, 2025

Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor

Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor

Published on: December 15, 2013

Repurposing Decellularized Lung to Generate Vascularized Fat.

Lindsey K Huff¹, Zihan Ling¹, Megan K DeBari²

¹Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|March 13, 2024

Summary

This summary is machine-generated.

Creating pre-vascularized adipose grafts using decellularized lung matrices and patient stem cells addresses a key surgical challenge. This innovative approach aims to improve reconstructive outcomes for subcutaneous adipose tissue defects.

Keywords:

Adipose tissue Adipose-derived stromal/stem cells Decellularized lung matrix Perfusion culture Reconstruction Vascularization

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Last Updated: Jul 1, 2025

Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor

Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor

Published on: December 15, 2013

Procedure for Lung Engineering

Procedure for Lung Engineering

Published on: March 8, 2011

Three-Dimensional Culture of Vascularized Thermogenic Adipose Tissue from Microvascular Fragments

Three-Dimensional Culture of Vascularized Thermogenic Adipose Tissue from Microvascular Fragments

Published on: February 3, 2023

Area of Science:

Biomaterials Science
Regenerative Medicine
Tissue Engineering

Background:

Reconstructing critical-sized subcutaneous adipose tissue defects is challenging due to the lack of pre-vascularized grafts.
Adipose tissue requires a dense microvasculature for nutrient/oxygen delivery and endocrine signaling.

Purpose of the Study:

To develop large-volume, perfusable, pre-vascularized adipose grafts.
To overcome the limitations of conventional adipose tissue reconstruction techniques.

Main Methods:

Combining decellularized lung matrices with patient-specific adipose-derived stem cells and endothelial cells.
Employing a step-wise cell seeding strategy within the matrix.

Main Results:

The study presents a novel method for creating vascularized adipose tissue constructs.
This technique facilitates the development of large-volume adipose grafts with potential for perfusion.

Conclusions:

This approach offers a promising solution for adipose tissue reconstruction by addressing the critical need for vascularization.
The combination of decellularized matrices and patient-derived cells represents a significant advancement in regenerative medicine for adipose defects.