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CD44-Based Detection of CSCs: CD44 Immunodetection by Flow Cytometry.
Lornella Seeneevassen1, Anissa Zaafour1, Christine Varon2
1INSERM U1312 BRIC Bordeaux Institute of Oncology, University of Bordeaux, Bordeaux, France.
Methods in Molecular Biology (Clifton, N.J.)
|March 13, 2024
Summary
This study details a flow cytometry protocol for detecting CD44, a marker for cancer stem cells (CSCs). This method precisely quantifies CD44 expression in various cancer samples.
Area of Science:
- Oncology
- Immunology
- Cell Biology
Background:
- CD44 is recognized as a significant marker for cancer stem cells (CSCs) across numerous malignancies.
- Accurate detection of CSCs is crucial for understanding cancer progression and developing targeted therapies.
Purpose of the Study:
- To provide a detailed protocol for detecting CD44 expression using flow cytometry.
- To enable precise quantification of CD44-positive cells and their expression levels.
Main Methods:
- The study outlines a flow cytometry protocol for CD44 detection.
- This technique is applicable to cell suspensions derived from both solid tissues and in vitro cell cultures.
Main Results:
- Flow cytometry allows for precise determination of the percentage of CD44-positive cells.
- The method also quantifies the mean fluorescent intensity, reflecting CD44 expression levels.
Conclusions:
- The described flow cytometry protocol is a reliable method for detecting CD44 in cancer research.
- This technique facilitates the characterization of cancer stem cells in various biological samples.
