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Adaptation of Semiautomated Circulating Tumor Cell CTC Assays for Clinical and Preclinical Research Applications
Published on: February 28, 2014
CD44-Based Detection of CSCs: CD44 Immunodetection by Flow Cytometry
Lornella Seeneevassen1, Anissa Zaafour1, Christine Varon2
1INSERM U1312 BRIC Bordeaux Institute of Oncology, University of Bordeaux, Bordeaux, France.
Abstract:
CD44 has been described in many malignancies as a marker of cancer stem cells (CSCs). Several techniques can be used to detect these cells. Here we detail CD44 detection by flow cytometry, a precise technique allowing to determine the percentage of positive cells and the mean fluorescent intensity reflecting the CD44 expression by cells in the samples. The protocol explained here can be used to detect CD44 from cell suspensions prepared from tissues or in vitro cell cultures.
