Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phases of Wound Repair01:28

Phases of Wound Repair

6.0K
Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
6.0K
Inflammation01:38

Inflammation

53.4K
Overview
53.4K
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

2.6K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
2.6K
Inflammatory Response01:28

Inflammatory Response

2.0K
An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
2.0K
Overview of Regeneration and Repair01:19

Overview of Regeneration and Repair

4.0K
Regeneration and repair processes are critical in healing damages caused by injury, disease, and aging. In regeneration, the damaged tissue is entirely replaced with new growth that restores the original architecture and function. In contrast, tissue repair usually results in a fixed tissue architecture involving scar formation. Scars generally do not reestablish tissue function and may also exhibit structural abnormalities at the injury site.
Regeneration
All animals have varying degrees of...
4.0K
Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

2.4K
Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...
2.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vanin-1 deficiency enhances host tolerance to influenza infection by modulating cellular redox status.

PNAS nexus·2026
Same author

Inadvertent Dose Reduction of Chemotherapy in a Patient Receiving Multiagent Neoadjuvant Chemotherapy.

JCO oncology practice·2026
Same author

Author Correction: Environmentally driven immune imprinting protects against allergy.

Nature·2026
Same author

On the balance of knowledge.

Nature reviews. Immunology·2026
Same author

Environmentally driven immune imprinting protects against allergy.

Nature·2026
Same author

The scavenger receptor MARCO is a ligand for the immune inhibitory receptor LAIR-1 and regulates its function in cis.

Science signaling·2025

Related Experiment Video

Updated: Jul 1, 2025

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces
07:55

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

Published on: January 7, 2020

7.4K

Mechanosensing regulates tissue repair program in macrophages.

Matthew L Meizlish1,2, Yoshitaka Kimura1, Scott D Pope1

  • 1Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.

Science Advances
|March 13, 2024
PubMed
Summary

Macrophages sense the extracellular matrix (ECM) mechanical properties through cytoskeletal remodeling. This integrin-independent, amoeboid-like sensing regulates tissue repair and gene expression, offering insights into fibrosis.

More Related Videos

Author Spotlight: Studying Macrophage-Epithelial Cell Interactions in Salivary Gland Regeneration After Injury
05:40

Author Spotlight: Studying Macrophage-Epithelial Cell Interactions in Salivary Gland Regeneration After Injury

Published on: November 17, 2023

1.5K
Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging
09:10

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging

Published on: July 16, 2021

3.2K

Related Experiment Videos

Last Updated: Jul 1, 2025

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces
07:55

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

Published on: January 7, 2020

7.4K
Author Spotlight: Studying Macrophage-Epithelial Cell Interactions in Salivary Gland Regeneration After Injury
05:40

Author Spotlight: Studying Macrophage-Epithelial Cell Interactions in Salivary Gland Regeneration After Injury

Published on: November 17, 2023

1.5K
Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging
09:10

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging

Published on: July 16, 2021

3.2K

Area of Science:

  • Cell Biology
  • Biophysics
  • Immunology

Background:

  • Tissue-resident macrophages are crucial for maintaining tissue homeostasis and repair.
  • The mechanisms by which macrophages monitor and regulate tissue integrity are not fully understood.
  • The extracellular matrix (ECM) provides structural support and organization to all tissues.

Purpose of the Study:

  • To investigate how macrophages sense the mechanical properties of the ECM.
  • To elucidate the molecular mechanisms underlying macrophage mechanosensing.
  • To understand the role of macrophage mechanosensing in regulating tissue repair programs.

Main Methods:

  • Utilized three-dimensional culture systems to mimic in vivo environments.
  • Investigated cytoskeletal remodeling dynamics in macrophages.
  • Examined integrin-independent mechanosensing pathways.
  • Analyzed the integration of biochemical signaling (e.g., colony-stimulating factor 1).
  • Assessed chromatin accessibility and gene expression changes.

Main Results:

  • Macrophages sense ECM mechanical properties via cytoskeletal remodeling.
  • Mechanosensing occurs in 3D environments through a noncanonical, integrin-independent mechanism resembling amoeboid migration.
  • Cytoskeletal dynamics integrate biochemical signals like colony-stimulating factor 1.
  • This process regulates chromatin accessibility and controls mechanosensitive gene expression.

Conclusions:

  • Macrophages employ an "amoeboid" mode of ECM mechanosensing.
  • This mechanism is critical for regulating specific tissue repair programs.
  • The findings provide insights into macrophage roles in tissue repair and potentially fibrosis.